碱性成纤维细胞生长因子对大鼠实验性肾小管损伤和间质病变的作用  

The role of bFGF in renal tubular injury and the interstitial lesion

作　　者：杜爱萍[1] 邹万忠[1] 

机构地区：[1]北京医科大学病理学系,100083

出　　处：《中华病理学杂志》2000年第3期208-211,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目!(39770293)

摘　　要：目的　研究碱性成纤维细胞生长因子 (bFGF)在大鼠肾小管损伤、再生以及肾间质病变过程中的作用。方法　应用Northern印迹杂交、原位杂交及免疫组织化学SP方法 ,观察在大鼠的庆大霉素中毒性肾小管损伤和再生过程中 ,bFGF及其受体 (FGFR1 )表达的情况 ,3 H TdR掺入法检测bFGF对培养的肾小管上皮细胞和肾间质成纤维细胞增殖的影响。结果　肾小管上皮细胞损伤和再生过程中 ,bFGFmRNA有表达 ,随着修复的增强 ,表达量逐渐增高 ;同时bFGF和FGFR1蛋白合成亦增加 ,主要分布于损伤及再生的肾小管上皮细胞 ,与mRNA分布一致。bFGF能够促进培养的肾小管上皮细胞和肾间质成纤维细胞的分裂增殖。结论　肾小管损伤和再生过程中 ,bFGF和FGFR1表达增强 ,一方面促进肾小管损伤的修复 ;另一方面还促进肾间质成纤维细胞增殖 。Objective The role of bFGF in renal tubular injury, regeneration and interstitial lesion of rats was studied. Methods The renal tubular injury model of rat was induced by intraperitoneal injection of gentamicin. The expression of bFGF and FGFR1 was examined by Northern blot, in situ hybridization and immunohistochemistry. 3H TdR incorporation was used to detect the role of bFGF in promoting the proliferation of renal tubular epithelial cells and renal fibroblasts. Results The expression of bFGF mRNA and protein synthesis of bFGF and FGFR1 increased during the process of injury and regeneration of tubular epithelial cells . In contrast, normal tubular epithelium failed to express bFGF, except renal interstitial fibroblasts and monocytes/macrophages. bFGF promoted proliferation of renal tubular epithelium as well as renal interstitial fibroblasts. Conclusion bFGF is secreted by tubular epithelial cells damaged by nephrotoxicity of gentamicin, and FGFR1 expressed in damaged renal tubules and interstitial fibroblasts. bFGF not only can promote tubular epithelia to proliferate, but can also stimulate interstitial fibroblasts to proliferate. Correlation exists between bFGF, renal tubular injury and interstitial lesions.

关 键 词：肾小管损伤 肾小管间质病变 BFGF 大鼠 

分 类 号：R363[医药卫生—病理学]