辣椒素致幼鼠特应性皮炎模型的建立  

Establishment of a juvenile rat model of atopic dermatitis by capsaicin

作　　者：孟祥慧[1] 初金玉[1] 陈丽丽[1] 

机构地区：[1]牡丹江医学院附属红旗医院皮肤科,黑龙江牡丹江157000

出　　处：《解剖科学进展》2012年第6期549-551,共3页Progress of Anatomical Sciences

摘　　要：目的探讨应用辣椒素建立一种新的特应性皮炎幼鼠动物模型。方法新生Wistar大鼠出生48h内背部皮下注射辣椒素(50mg/kg),3w后观察记录幼鼠的搔抓行为和皮损表现,苏木精-伊红染色观察病损皮肤组织病理变化,应用ELISA技术测定幼鼠血清中白介素(IL)-13和免疫球蛋白(Ig)E的水平,同时采用蛋白印迹法研究病损皮肤组织中丝聚合蛋白和神经生长因子的表达变化。结果与对照相比,新生鼠辣椒素皮下注射不仅引起严重瘙痒和皮损表现,而且有明显表皮增生和肥大细胞浸润等病理变化,ELISA结果显示模型鼠血清中IL-13和IgE水平增高,皮损组织中神经生长因子表达显著增加,而丝聚合蛋白表达明显降低,说明皮肤屏障功能受损。结论辣椒素皮下注射的幼鼠模型具有人类特应性皮炎患儿类似的临床表现和病理生理变化,该模型可以为研究儿童特应性皮炎的发病机制和治疗打下基础。Objective To establish an animal model of atopic dermatitis（AD） in juvenile Wistar rats by capsaicin. Methods Newborn rats were injected subcutaneously with capsaicin（50mg/kg） within 48h after birth, and scratching behavior and dermatitis manifestations in juvenile rats were observed three weeks later. Pathological changes were investigated by HE staining in juvenile rat skin. The levels of interleukin （IL）-13 and immunoglobulin （Ig） E in serum were determined by ELISA technique. Meanwhile, the levels of filaggrin and nerve growth factor protein in juvenile rat skin were detected by Western blot. Results Compared with the control, capsaicin injection in newborn rats led to severe scratching and cutaneous lesions, obvious histopathological changes including epidermal hyperplasia and mast cells infiltration were observed, serum levels of IL-13 and IgE were higher by ELISA, the expression level of nerve growth factor in skin lesions was notably increased,whereas, filaggrin expression level was significantly reduced in model group, indicating impaired skin barrier function. Conclusion Juvenile rat AD model was established successfully by capsaicin and had similar clinical manifestations and pathophysiological changes to AD children. This model may provide the basis for the research on the pathogenesis and therapy of children atopic dermatitis.

关 键 词：辣椒素 新生Wistar大鼠 特应性皮炎 丝聚合蛋白 神经生长因子 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]