重组人促红细胞生成素对SCI大鼠Akt和p-Akt表达的影响  

The effect of recombinant human erythropoietin on the expression of Akt and p-Akt in spinal cord injury rat

作　　者：车敏[1] 刘颖[2] 王岩峰[1] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁沈阳110001 [2]中国医科大学附属第一医院手术室,辽宁沈阳110001

出　　处：《解剖科学进展》2012年第6期556-559,563,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.81100924);辽宁省教育厅科研基金资助项目(L2010687)

摘　　要：目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rHuEPO)对脊髓损伤(spinal cord injury,SCI)大鼠Akt与p-Akt表达的影响。方法成年健康Wistar大鼠72只,雌雄不限,按随机数字表法分为假手术组、脊髓损伤组和重组人促红细胞生成素治疗组,参照Nystrom's压迫方法制作大鼠脊髓压迫损伤模型,按照存活时间再分为脊髓损伤6h,12h,24h,3d组。免疫组化和Western blot方法检测Akt、p-Akt在各组大鼠脊髓表达的变化。结果免疫组化和Westernblot结果发现,脊髓Akt、p-Akt蛋白阳性表达的平均光密度值SCI组低于假手术组(P<0.05),重组人促红细胞生成素治疗组显著高于SCI组(P<0.05)。结论 rHuEPO可通过上调Akt、p-Akt蛋白的表达参与脊髓损伤修复。Objective To study the effect of recombinant human erythropoietin（rHuEPO） on the expressions of Akt and p-Akt in spinal cord injury rat. Methods 72 healthy adult Wistar rats were randomly divided into the sham group（n=8）, the spinal cord injury group（n=32） and rHuEPO group（n=32）. The spinal cord injury was induced with Nystrom＇s way. The spinal cord injury group and rHuEPO group were further randomly subdivided into four subgroups （6h, 12h, 24h, 3d） according to the postoperative survival time. The expressions of Akt and p-Akt were observed by immunochemistry and Western blot methods. Results The mean optic density（MOD） values of Akt and p-Akt products in spinal cord were decreased in spinal cord injury than in sham group（ P0.05）, but increased significantly in rHuEPO treated group than in spinal cord injury（ P0.05） by immunochemistry and Western blot. Conclusion The impairment effect of rHuEPO in spinal cord injury might be related to the upregulation of the expressions of Akt and p-Akt.

关 键 词：脊髓损伤 重组人促红细胞生成素 AKT P-AKT 大鼠 

分 类 号：R744[医药卫生—神经病学与精神病学] R322.121[医药卫生—临床医学]