脑络欣通对局灶性脑缺血－再灌注大鼠血液流变学及相关调节因子的作用  

Effects of naoluoxintong on hemorheology and related regulatory factors in focal cerebral ischemia-reperfusion rats

作　　者：王键[1] 谭辉[1] 菅威[1] 胡建鹏[1] 何玲[1] 李祥[1] 

机构地区：[1]安徽中医学院细胞分子生物学脑病实验室省部共建新安医学教育部重点实验室,合肥230038

出　　处：《中国脑血管病杂志》2012年第11期590-594,共5页Chinese Journal of Cerebrovascular Diseases

基　　金：国家科技支撑计划(2012BAI26B00);安徽省科技厅资助项目(100432);康缘中医药科技创新基金项目(KYCX201010)

摘　　要：目的观察脑络欣通对大脑中动脉闭塞缺血-再灌注(MCAO/R)大鼠血液流变学及相关调节因子的影响。方法将Wistar大鼠60只,随机分为假手术组、模型组、通心络组、脑络欣通低剂量(40 mg/ml)组、中剂量(80 mg/ml)组及高剂量(100 mg/ml)组,每组各10只。采用线栓法制备大脑中动脉闭塞缺血-再灌注(MCAO/R)模型。采用全自动血液流变仪检测全血黏度、血浆黏度、红细胞比容(HCT);高性能血凝仪检测血浆纤维蛋白原(FIB)含量;运用双抗体一步夹心法酶联免疫吸附实验,检测血小板生成素(TPO)、内皮素(ET)水平;运用激光多普勒仪,于造模后第14天监测各组大鼠的局部脑血流量(rCBF)。结果①假手术组、通心络组、脑络欣通3个剂量组大鼠的全血黏度各项、血浆黏度、HCT和FIB指标均低于模型组,差异有统计学意义,P<0.01。②通心络组、脑络欣通中剂量和高剂量组的HCT低于脑络欣通低剂量组,差异有统计学意义,P<0.01。③假手术组、通心络组及脑络欣通3个剂量组大鼠的TPO、ET水平均低于模型组,rCBF高于模型组,差异具有统计学意义,P<0.01或P<0.05。④脑络欣通中剂量组大鼠TPO水平低于脑络欣通低剂量组,rCBF高于脑络欣通低剂量组,差异具有统计学意义,P<0.05。结论脑络欣通可以改善MCAO/R模型大鼠的血液流变学指标,调节凝血纤溶系统相关因子,对缺血性脑血管病可能具有良好的作用。Objective To observe the effects of Naoluoxintong on hemorheology （rCBF） and related regulatory factors in rats with middle cerebral artery occlusion-reperfusion （MCAO/R） . Methods Sixty Wistar rats were randomly divided into sham operation, model, tongxinluo, naoluoxintong 40 mg,/ml, Naoluoxintong 80 mg/ml, and Naoluoxintong 100 mg/ml groups. A rat model of MCAO/R was induced by the intraluminal suture method. Whole blood viscosity,plasma viscosity and hematocrit （HCT） were detected by an automatic hemorheology analyzer. The content of fibrinogen （FIB） was detected by a high performance coagulation analyzer. The levels of thrombopoietin （TPO） and endothelin （ET） were detected by double antibody sandwich enzyme-linked immunosorbent assay （ELISA）. The rCBF of the rats was detected by laser Doppler f/owmetry at the 14th day after modeling in each group. Results ①Whole blood viscosity, plasma viscosity, HCT, and FIB in the sham operation, Tongxinluo and Naoluoxintong groups were all lower than those in the model group. The differences were statistically significant （ P 〈 0.01 ）. ②HCT in the Tongxinluo, Naoluoxintong 80 mg/ml and Naoluoxintong 100 mg/ml groups was lower than that in the Naoluoxintong 40 mg/ml group. The differences were statistically significant （ P 〈 0.01 ）. ③The levels of TPO and ET in the sham operation,Tongxinluo and 3 Naoluoxintong groups were lower than those in the modelgroup, but the rCBF was higher than that in the model group. The differences were statistically significant （ P 〈 0.01 or P 〈 0.05 ）. ④The level of TPO in the naoluoxintong 80 mg/ml group was lower than that in the naoluoxintong 40 mg/ml group, but the rCBF was higher than that in the naoluoxintong 40 mg/ml group. The difference was statistically significant （ P 〈 O. 05 ）. Conclusion Naoluoxintong can improve the hemorheology indexes and regulate the relevant factors of the fibrinolysis coagulation system in MCAO model rats ,which has a good effect on ischemic cerebr

关 键 词：血液流变学 血小板生成素 脑络欣通 内皮素 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]