酶法合成5-氟尿苷  

Enzymatic Synthesis of 5-Fluorouridine with Enterobacter Aerogenes

作　　者：雷霆[1] 周长林[1] 窦洁[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生物制药学院,南京210009

出　　处：《药物生物技术》2000年第2期102-105,共4页Pharmaceutical Biotechnology

摘　　要：采用产气肠杆菌 (Enterobacteraerogenes)CPU 785 6变异株的游离细胞 ,利用其中的嘧啶核苷磷酸化酶 (UridinePhosphorylase)通过碱基交换进行酶法合成目的物。试验以 30mmol/L尿苷 (UR) ,30mmol/L 5 氟尿嘧啶 (FU) ,30mmol/L磷酸盐缓冲液 (pH7 8)的混合物加入 10 %的产气肠杆菌湿菌体反应 3h ,底物UR的转化率可达 5 0 %。The enzymatic synthesis of 5 fluoro uridine with Enterobacter aerogenes nucleoside phosphorylase was studied The substrates of it were 5 fluorouracil,uridine and potassium phosphate The experiment showed that the optimum substrates concentration was 30mmol/L UR,30mmol/L FU,25 30mmol/L potassium phosphate When we added 10% (W/V) E. aerogenes wet cell cultured for 24h to the reacting mixture and adjusted pH to 7 0 with KOH,the biosynthesis took place at 65℃ for 3h effectively and 50% UR was converted to FUR

关 键 词：S-氟尿苷 酶活合成 嘧啶核苷磷酸化酶 

分 类 号：R914.5[医药卫生—药物化学]