Hippo信号通路在胃黏膜癌变过程中的作用  

Expression of Hippo signaling molecules in gastric carcinogenesis

作　　者：魏文超[1] 张翠萍[1] 张琪[1] 王小玮[1] 张红珠[1] 赵坤[1] 田字彬[1] 

机构地区：[1]青岛大学医学院附属医院消化科,山东省青岛市266003

出　　处：《世界华人消化杂志》2012年第31期3027-3032,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨Hippo信号通路的核心组件Mst1、Lats1及Yap1在正常组、慢性萎缩性胃炎伴肠上皮化生、不典型增生、胃癌组中的表达及临床意义.方法:应用PV-6000两步免疫组织化学法测定Hippo通路中3个核心组件Mst1、Lats1及Yap1在30例正常胃黏膜,60例肠上皮化生、不典型增生及胃癌胃黏膜中的表达情况.结果:Mst1、Lats1在胃癌中的阳性表达率明显低于其余各组,其中Mst1在胃癌组与正常组,肠上皮化生组,不典型增生组中阳性表达的差异有明显统计学意义(P分别为0.000、0.001和0.007),而Lats1在胃癌组与正常组,肠上皮化生组,不典型增生组中阳性表达也有显著差异(P分别为0.006、0.002和0.004).由正常组至不典型增生组,Mst1、Lats1的阳性表达率呈逐渐降低的趋势;Yap1在胃癌中的阳性表达率明显高于其余3组(P分别为0.000、0.002和0.006),差异有统计学意义;Yap1由正常组至不典型增生组其阳性表达率呈逐渐增高的趋势;Mst1、Lats1及Yap1的阳性表达率与胃癌分化程度及有无淋巴结转移密切相关,差异有显著统计学意义(P分别为0.007、0.028;0.007、0.014;0.009、0.013);胃癌组织中,Yap1与Mst1、Lats1的表达呈负相关(r分别为-0.715、-0.642),Mst1与Lats1的表达呈正相关(r=0.702).结论:Hippo信号通路在胃癌的发生发展中起着重要作用,其转录共激活因子Yap可能会成为胃癌治疗中新的靶点.AIM：To observe the expression of Mst1,Lats1 and YAP1 in normal gastric mucosa,chronic atrophic gastritis with intestinal metaplasia,dys-plasia and gastric cancer.METHODS：Immunohistochemistry was used to investigate the expression of Mst1,Lats1 and YAP1 in 30 cases of normal gastric musoca,60 cases of chronic atrophic gastritis with intestinal metaplasia,60 cases of dysplasia,and 60 cases of gastric cancer.RESULTS：The positive rates of Mst1 and Lats1 expression in gastric cancer were significantly lower than those in the normal control group,intestinal metaplasia group,and dysplasia group（Mst1：P = 0.000,0.001,0.007;Lats1：P = 0.006,0.002,0.004）.Mst1 and Lats1 expression showed a decreasing trend from the normal gastric mucosa to dysplasia.The positive rate of Yap1 expres-sion in gastric cancer was significantly higher than those in the normal control group,intestinal metaplasia group,and dysplasia group（P = 0.006,0.002,0.004）,and Yap1 expression showed an increasing trend from the normal gastric mucosa to dysplasia.The positive rates of Mst1,Lats1 and Yap1 expression had a close correlation with tu-mor differentiation and lymph node metastasis（P = 0.007,0.028,0.007;P = 0.014,0.009,0.013）.The positive rate of Yap1 expression had a negative correlation with those of Mst1 and Lats1 expres-sion in gastric caner（r =-0.715,-0.642）,while Mst1 expression had a positive correlation with Lats1 expression（r = 0.702）.CONCLUSION：The Hippo signaling pathway may play an important role in the development of gastric cancer.

关 键 词：胃癌 Hippo通路 抑癌基因 癌基因 胃黏膜癌变 

分 类 号：R735.2[医药卫生—肿瘤]