E-cadherin、β-catenin在儿童幼年性息肉中的表达及意义  

Signifi cance of expression of E-cadherin and-catenin in juvenile colorectal polyps in children

作　　者：金美芳[1] 朱雪明[1] 顾红英[2] 王兴东[3] 刘琳[1] 张闽[3] 李晓未[3] 

机构地区：[1]苏州大学附属儿童医院儿研所,江苏省苏州市215003 [2]苏州大学附属儿童医院消化内科,江苏省苏州市215003 [3]苏州大学附属儿童医院病理科,江苏省苏州市215003

出　　处：《世界华人消化杂志》2012年第31期3051-3056,共6页World Chinese Journal of Digestology

基　　金：苏州市科技发展计划基金资助项目;No.SZD09116~~

摘　　要：目的:分析E-cadherin、β-catenin在幼年性息肉(juvenilepolyp,JP)中的表达及在细胞内的分布,探讨二者在儿童JP形成的作用.方法:采用荧光定量PCR和Western blot检测22例JP组织及8例正常肠组织中E-cadherin、β-catenin mRNA和蛋白的表达.免疫组织化学法检测30例JP组织和10例正常组织中二者的表达状态及分布.结果:E-cadherin mRNA在JP组织中的表达较正常对照明显下调(1.1526±0.3801vs0.4094±0.2305,P<0.05);β-catenin mRNA在JP组与正常组中的表达则无差异(1.7084±0.7467vs1.9428±0.8111,P>0.05).Westernblot结果显示E-cadherin较正常组低(0.6028±0.1778vs0.3257±0.1168,P<0.05);-catenin在JP组中的表达则上调(0.8010±0.1380vs1.2064±0.3587,P<0.05).免疫组织化学分析结果与Western blot一致,E-cadherin在JP组织中的表达较正常对照组明显下调(102.5155±25.2988vs53.5772±15.3205,P<0.05),阳性染色主要见于上皮细胞膜;-catenin在JP组织中膜表达比正常对照组明显上升(112.0805±24.9572vs260.3554±86.6987,P<0.05),且在JP组织上皮细胞胞浆及核内亦见阳性着色.结论:JP组织中E-cadherin的表达下调,而β-catenin蛋白表达则上调,且分布发生改变,二者表达的异常可能是JP的形成的特征之一.AIM：To detect the expression of E-cadherin and-catenin mRNAs and proteins in human colorectal juvenile polyps（JP） in children.METHODS：The expression of E-cadherin and-catenin mRNAs and proteins in 22 juve-nile colorectal polyp specimens and 8 normal colorectal specimens was assayed by real-time PCR and Western blot,respectively.Immunohis-tochemistry was used to determine the expres-sion and localization of E-cadherin and-catenin in 30 juvenile colorectal polyp specimens and 10 normal colorectal specimens.RESULTS：Real-time PCR and Western blot analyses showed that the expression levels of E-cadherin mRNA and protein were signifi-cantly lower in juvenile colorectal polyps than in normal colorectal specimens（1.1526 ± 0.3801 vs 0.4094 ± 0.2305,P 0.05;0.6028 ± 0.1778 vs 0.3257 ± 0.1168,P 0.05）.Although there was no significant difference in the expression level of-catenin mRNA between the two groups,the expression of-catenin was signif icantly higher in juvenile colorectal polyps than in normal colorectal specimens（0.8010 ± 0.1380 vs 1.2064 ± 0.3587,P 0.05）.Immunohistochemistry in-dicates that E-cadherin expression was down-regulated in juvenile polyps compared with controls（102.5155 ± 25.2988 vs 53.5772 ± 15.3205,P 0.05）.Positive staining for E-cadherin was predominantly localized on the epithelial cell membrane.The expression of-catenin was in-creased in juvenile polyp specimens（112.0805 ± 24.9572 vs 260.3554 ± 86.6987,P 0.05）,and the positive rates of membrane,cytoplasmic and nuclear staining in juvenile polyp tissue was signifi cantly higher than those in normal control tissues.CONCLUSION：Dysregulation of E-cadherin and-catenin expression may be an important feature of juvenile colorectal polyps.

关 键 词：幼年性息肉 上皮钙粘附蛋白 Β连环素 

分 类 号：R730.2[医药卫生—肿瘤]