蜕皮甾酮对大鼠脑组织自由基损伤的保护作用  

Ecdysterone protects brain injury against free radical in rats

作　　者：高诗豪[1] 唐政恒[1] 陈图南[1] 李飞[1] 单佑安[1] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科,全军神经外科研究所,全军神经创伤防治重点实验室,重庆400038

出　　处：《第三军医大学学报》2012年第23期2357-2359,共3页Journal of Third Military Medical University

摘　　要：目的观察蜕皮甾酮(ecdysterone,EDS)对大鼠脑损伤的治疗作用。方法 SD雄性大鼠120只,按随机数字表法分6组,每组20只。采用Feeney法自由落体打击鼠脑造模。假手术组动物只开颅,不打击;治疗组:分别采用4、8、16 mg/kg的EDS腹腔注射模型动物;阴性治疗对照组:模型动物腹腔注射生理盐水;阳性治疗对照组:模型动物腹腔注射3 mg/kg依达拉奉。伤后7 d计算各组动物死亡率;检测各组动物脑组织含水量及丙二醛(MDA)、羟自由基能力及超(过)氧化物歧化酶(superoxide dismutase,SOD)等。HE染色观测各组大鼠脑组织海马病变情况。结果伤后7 d,16 mg/kg EDS治疗组的死亡率、脑组织含水量MDA与羟自由基含量较阴性治疗对照组明显降低(P<0.05),SOD活性显著升高(P<0.01),脑细胞损害明显减轻,大鼠海马CA1区正常神经元数量明显优于阴性治疗组(P<0.01)。结论蜕皮甾酮对脑损伤具有保护治疗作用。Objective To investigate the effect of ecdysterone （EDS） on traumatic brain injury （TBI） in rats. Methods A total of 120 male Sprague-Dawley rats were randomly and equally divided into 6 groups, sham-operation group, TBI group, edaravone treatment group and EDS treatment groups. Modified Feeney＇ s method was used to establish the rat model of experimental TBI with an impact of a weight of 30 g from 20 cm after craniotomy. Rats of EDS treatment groups were given EDS at 4, 8, and 16 mg/kg via perito- neal injection for 7 d. The other 3 groups were treated with normal saline or edaravone （3 mg/kg）. The animal death rate was calculated, and the changes of the brain water content, malondialdehyde （ MDA）, and superox- ide dismutase （SOD）in brain tissue were calculated and measured. Hematoxylin-eosin （HE） staining was used to observe the pathological changes of brain hippocampus tissue. Results EDS （ 16 mg/kg） resulted in significant decreases in the death rate, brain water content, and MDA content （P 〈 0.05 ）, and an obvious increase in SOD content （P 〈0.01） compared with TBI group. EDS （16 mg/kg） also resulted in lesser damage in the brain cells, with the pyramidal cells number significantly higher than TBI group （ P 〈 0.01 ）. Conclusion EDS exerts therapeutic effect on TBI through inhibiting free radical damage and brain edema.

关 键 词：蜕皮甾酮 脑损伤 死亡率 脑水肿 自由基 

分 类 号：R362[医药卫生—病理学] R651.15[医药卫生—基础医学]