VM-26诱导胶质母细胞凋亡的实验研究  

Apoptosis of glioblastoma multiforme cells induced by VM-26 treatment in vitro

作　　者：赵承军[1] 孔繁元 胡庆和[1] 吴若芬[2] 

机构地区：[1]宁夏医学院中心实验室,宁夏银川750004 [2]宁夏医学院附属医院中心实验室,宁夏银川750004

出　　处：《癌症》2000年第7期649-652,共4页Chinese Journal of Cancer

摘　　要：目的：选用体外培养人多形性胶质母细胞BT325作为对象，研究抗癌药物VM－26的治疗作用机理。方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。结果：实验显示VM－26作用于体外培养人多形性胶质母细胞BT325后可诱导其发生凋亡。结论：VM-26抑制体外培养BT325细胞的生长，是通过阻滞细胞周期的进程，启动细胞自身的调控程序，诱导细胞凋亡，从而达到抗肿瘤的作用。Objective: The purpose of this experiment is to investigate the mechanism of therapeutic effect of anticancer drug teniposide on cultured human glioblastoma multiforme cells BT325. Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis. Result: The results of this experiment indicate that the therapeutic effect of VM-26 on BT325 cell was induced by apoptosis. Conclusion: The mechanism of apoptosis is involved in the inhibition of BT325 cell growth caused by VM-26 treatment.

关 键 词：细胞凋亡 胶质母细胞 VM-26 抗癌药 

分 类 号：R730.2[医药卫生—肿瘤]