头颈部鳞癌及癌旁组织端粒酶活性研究  

Telomerase activity in human head and neck squamous cell carcinoma and adjacent tissues

作　　者：陶泽璋[1] public.wh.hb.cn 刘剑锋[1] 肖伯奎[1] 杨强[1] 詹汉章[1] 

机构地区：[1]湖北医科大学附属第一医院耳鼻喉头颈外科

出　　处：《癌症》2000年第7期671-673,共3页Chinese Journal of Cancer

摘　　要：目的：研究原发头颈部鳞癌及相应癌旁组织中端粒酶活性表达，并探讨其作为头颈部鳞癌分子生物学标志物的可能性。方法：采用TRAP－PCR－ELISA法对32例原发头颈部鳞癌及15例相应癌旁组织进行端粒酶活性的定性和定量检测。结果：原发头颈部鳞癌组端粒酶活性值明显高于癌旁组（P＜0．05）。低分化组端粒酶活性值明显高于中高分化组（P＜0．01）。Ⅰ、Ⅱ与Ⅲ期端粒酶活性值有显著差异（P＜0．05）。鼻咽组端粒酶活性值明显高于下咽组（P＜0．05）。淋巴结阳性组端粒酶活性值高于淋巴结阴性组，但无统计学意义。癌和癌旁组织均为阳性者，癌和癌旁组织端粒酶活性值无差异（P＞0．05）。结论：端粒酶活化与头颈部鳞癌的发生发展有密切关系，其活化是早期事件，与临床指标关系密切，可望成为头颈部鳞癌早期诊断、治疗和判断预后的分子生物学标志物。Objective: To investigate the telomerase activity in human head and neck squamous cell carcinomas and their adjacent tissues and to explore the possibility of telomerase activity as tumor biological marker. Methods: Thiriy-two samples of primary head and neck squamous cell carcinoma and 15 adjacent tissues were assayed using TRAP-PCR-EUSA for detection of telomerase activity in quantity and quality. Results: The telomerase activity value in head and neck squamous cell carcinoma was higher than that in their adjacent tissues (P < 0. 01 ). The value of telomerase activity in poor differentiation cancer was higher than that in moderate and high grading cancer (P < 0. 01 ). A statistically significant difference was observed in telomerase activity value for Ⅰ and Ⅱ stage cancers compared with Ⅲ stage cancers (P <0. 05 ). There was significant difference of the telomerase activity between the site of nasopharynx and hypopharynx. No significant difference was found between the values of cancer and their adjacent tissues for those samples that were positive both in cancer and their adjacent tissues. The value of telomerase activation was lower in cancer without lymph node involvement than that with lymph node involvement, but the difference was not significancte (P > 0. 05 ). Conclusions: telomerase activation is consistent with the concept of telomerase playing a key role in tumorigenesis of head and neck squamous cell carcinoma. Activation of telomerase is an early event of tumorigenesis. Further study is needed to determine the usefulness of this enzyme as a new molecular marker for early clinical diagnosis, treatment modality selection and prognosis judgement.

关 键 词：头颈部鳞癌 端粒酶 癌旁组织 活性 

分 类 号：R739.91[医药卫生—肿瘤]