魔芋葡甘聚糖/透明质酸共混膜的体外细胞生物相容性  

In vitro cellular biocompatibility of konjac glucomannan-hyaluronic acid blend membrane

作　　者：顾邵[1] 潘险峰[1] 徐永清[1] 陈佳[1] 陈庆华[1] 

机构地区：[1]解放军成都军区昆明总医院骨科,昆明医科大学临床教学医院,云南省昆明市650032

出　　处：《中国组织工程研究》2012年第43期8055-8060,共6页Chinese Journal of Tissue Engineering Research

基　　金：中国博士后科学基金项目(20090461495)~~

摘　　要：背景:高分子材料透明质酸与魔芋葡甘聚糖均可用于防治术后粘连。目的:观察兔骨髓间充质干细胞与魔芋葡甘聚糖-透明质酸共混膜复合培养的生物相容性。方法:取第3代兔骨髓间充质干细胞与魔芋葡甘聚糖-透明质酸共混膜体外复合培养,倒置显微镜和扫描电镜观察复合程度,MTT法检测细胞增殖。体外定向外诱导魔芋葡甘聚糖-透明质酸共混膜上的骨髓间充质干细胞向脂肪细胞分化,油红O染色检测其分化效果。结果与结论:骨髓间充质干细胞与魔芋葡甘聚糖-透明质酸共混膜修复材料复合良好,倒置显微镜和扫面电镜观察均可见细胞在共混膜上良好黏附与增殖;体外定向诱导魔芋葡甘聚糖-透明质酸共混膜上的骨髓间充质干细胞可向脂肪细胞分化,具有成脂潜能。说明魔芋葡甘聚糖-透明质酸共混膜与骨髓间充质干细胞具有良好的生物相容性。BACKGROUND： Hyaluronic acid and konjac glucomannan can be used for the prevention of adhesion after surgery OBJECTIVE： To investigate the biocompatibility of konjac glucomannan-hyaluronic acid blend membrane co-cultured with bone marrow mesenchymal stem cells （BMSCs） of rabbits. METHODS： The passage 3 BMSCs were cultured with konjac glucomannan-hyaluronic acid blend membrane in vitro, Their compound degree was observed by inverted microscope and scanning electron microscopy. Cell proliferation was detected by MTT assay. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane were orientiatedly induced into adipocytes in vitro. Differentiation effect was detected by oil red O staining RESULTS AND CONCLUSION： BMSCs were well cultured with konjac glucomannan-hyaluronic acid blend membrane Good adhesion and proliferation was found under inverted microscope and scanning electron microscope. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane could be orientiatedly induced into adipocytes, and had odipogenic potential. These findings suggest that there is a good biocompatibility between BMSCs and the konjac glucomannan-hyaluronic acid blend membrane,

关 键 词：魔芋葡甘聚糖 透明质酸 骨髓间充质干细胞 脂肪细胞 硬膜 粘连 生物相容性 生物材料 

分 类 号：R318[医药卫生—生物医学工程]