局灶脑缺血后胰岛素对c-fos、c-jun基因表达的影响  

Insulin effect on c-fos and c-jun gene expression in focal ischemic rat brain

作　　者：王艺东[1] 黄如训[2] 李玲[2] 曾木圣[3] 邢诒刚[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院神经科,广东广州510120 [2]中山医科大学附属第一医院神经科,广东广州510080 [3]中山医科大学肿瘤研究所,广东广州510060

出　　处：《中风与神经疾病杂志》2000年第3期144-146,共3页Journal of Apoplexy and Nervous Diseases

基　　金：广东省医药卫生青年科研基金资助课题!( 960 70 )

摘　　要：目的　观察胰岛素对局灶缺血性脑组织 c- fos、c- jun基因表达的影响。方法　 30只肾血管性高血压大鼠随机分 4组 ,在大脑中动脉闭塞 (MCAO)后即予胰岛素 (2 .1IU / kg,A组 )、葡萄糖和胰岛素 (2 .1IU / kg,B组 ;4.5 IU / kg,C组 )、生理盐水 (D组 ) ,采用原位杂交技术检测各组 MCAO后 3h脑组织 c- fos、c- jun基因的表达。结果　与其它组比较 ,A组血糖下降明显 (P<0 .0 1) ,MCAO后 1h、2 h、3h分别为 3.80± 0 .3mm ol/ L、3.6 8± 0 .3mm ol/L、3.6 9± 0 .3mm ol/ L。 c- fos m RNA被诱导出现于缺血侧皮层和尾壳核 ,C组 c- fos m RNA明显增加 (P<0 .0 1) ,皮层和尾壳核的灰度为 12 3.5 8± 8.72、141.0 8± 8.49;c- jun m RNA仅出现于缺血侧皮层 ,A、B、C组的 c- jun m RNA均增加明显 ,灰度分别为 171.82± 7.33、172 .5 6± 7.91、15 5 .0 9± 7.91,其中 C组的增加更为显著 (P<0 .0 1)。结论　胰岛素促进缺血脑组织 c- fos、c- jun基因表达可能是其神经保护作用机制之一。Objective To investigate the effect of insulin on c fos and c jun gene expression in focal ischemic rat brain. Methods The renovascular hypertensive rats (n=30) were divided at random into 4 groups which received separately with insulin only (Group A,2.1 IU/kg),insulin and glucose (Group B with low dose insulin,2.1 IU/kg;Group C with high dose insulin,4.5 IU/kg)or normal saline(Group D)immediately after middle cerebral artery occlusion (MCAO). Level of c fos mRNA and c jun mRNA on brain sections was measured by in situ hybridization at 3h after MCAO. Results Blood glucose level in group A was 3.80±0.3,3.68±0.3 and 3.69±0.3mmol/L at 1,2 and 3 hours following MCAO,which was lower than in the other groups(P<0.01). In ipsilateral cortex and caudoputamen after MCAO,c fos mRNA significantly increased only in group C(P<0.01) and c fos mRNA greg was 123.58±8.72 and 141.08±8.49. A significant increase in c jun mRNA in ipsilateral cortex was detected in Group A,B and C,and the strongest experssion was seen in Group C(P<0.01). c jun mRNA greg in Group A,B and C was 171.82±7.33、172.56±7.91 and 155.09±7.91. Conclusions These results indicate that the effect of insulin on c fos and c jun expression may be one of the mechanisms of neuroprotection of insulin.

关 键 词：胰岛素 局灶脑缺血 C-FOS基因 C-JUN基因 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]