不同牵张力对成骨细胞增殖与合成功能的影响  

The effect of stretch force on the proliferation and synthetic function of osteoblasts

作　　者：韩磊[1] 李煌[1] 赵计林[1] 唐国华[2] 

机构地区：[1]南京大学医学院附属口腔医院.口腔正畸科,210025 [2]上海交通大学医学院附属第九人民医院口腔正畸科.上海市口腔医学重点实验室

出　　处：《实用口腔医学杂志》2012年第6期677-681,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81070807)

摘　　要：目的:观察周期性牵张应变对成骨细胞增殖与合成功能的影响。方法:体外分离和培养新生大鼠颅顶骨成骨细胞,应用Flexcell 4000TM加力系统施加力学刺激,频率为0.5 Hz,牵张应变大小分别为1.5%、3%、6%及9%,作用时间分别为12、24及48 h。检测成骨细胞的增殖以及合成功能的变化。采用SAS 8.0软件包对结果进行统计分析。结果:①1.5%、3%的牵张应变可以促进成骨细胞I型胶原、骨钙素和总蛋白的分泌量增加,以3%牵张应变增加最为明显。同时,在3%牵张应变的初期可以促进成骨细胞的增殖,而促进成骨细胞的分化成熟的作用大于促进增殖的作用;②6%牵张应变下,成骨细胞骨钙素、I型胶原和总蛋白的分泌量减少,但在6%牵张应变作用的各时间段均使细胞增殖活性明显增高;③9%牵张应变下,成骨细胞增殖和分化功能均受抑制。结论:适度的牵张应变可促进成骨细胞的生长,较小的牵张应变促进分化成熟的作用较明显,较大的牵张应变促进增殖的作用较明显,但过大的牵张应变对细胞的增殖和分化均有抑制。Objective： To investigate the effects of mechanical tensile strain on the proliferation and synthetic function of osteoblasts. Methods： In vitro cuhured osteoblasts from newborn rat calvarial bone were subjected to 1.5% , 3%, 6% and 9% elongation of ten- sile stretche using Flexce11-4000TM strain unit at a frequency of 0.5 Hz for 4, 8 and 24 hours respectively. The proliferation of osteo- blasts was quantified by cell cycle detection via FCM. The synthetic function was studied by measuring PCIP, BGP and total protein in the culture supernatant. Statistical analysis was performed using SAS 8.0 software package. Results： The mechanical strain elevated the proliferation and synthetic function of osteoblasts by 3 % elongation. However, the mechanical strain increased the proliferation and suppressedthe synthetic function of the osteoblasts by 6% elongation. 9% elongation decreased the proliferation activity and the syn- thetic function of the cells. Conclusion： Moderate stretch can promote the proliferation of osteoblasts. Minor stretch can elevate the the synthetic function, while major stretch can promote the proliferation activity, over stretch may inhibite the proliferation and synthetic function of the cells.

关 键 词：成骨细胞 牵张力 增殖 

分 类 号：R782.2[医药卫生—口腔医学]