脐血单个核细胞移植治疗痉挛型小儿脑性瘫痪的安全性  

Transplantation of umbilical cord mononuclear cells for treatment of infantile spastic cerebral palsy

作　　者：徐蓉[1] 刘波[2,3] 段答[2,3] 樊梦瑶[2,3] 刘斌[1] 滕晓华[2,3] 赵振宇[2,3] 卢明[2,3] 

机构地区：[1]湖南师范大学医学院,湖南省长沙市410006 [2]湖南师范大学第二附属医院 [3]解放军第163医院神经外科,湖南省长沙市410003

出　　处：《中国组织工程研究》2012年第41期7787-7790,共4页Chinese Journal of Tissue Engineering Research

基　　金：湖南省科技厅项目(2008FJ3061)~~

摘　　要：背景:大量研究证实脐血间充质干细胞体外培养可被诱导分化为表达神经元和神经胶质细胞表面标志物的细胞,对神经系统疾病起到替代修复的作用。目的:观察人脐血单个核细胞移植治疗痉挛型小儿脑性瘫痪的疗效和安全性。方法:收集正常足月剖腹产胎儿的脐血,经肝素抗凝,用淋巴细胞分离液分离脐血单个核细胞,采用差速贴壁法进行纯化,并经腰椎穿刺注入30例痉挛型脑性瘫痪患者体内。结果与结论:移植后患者肢体的肌肉痉挛程度和关节活动度明显改善,与移植前相比差异有显著性意义(P<0.05),移植后和随访过程中无不良反应和并发症。BACKGROUND：A large number of studies have demonstrated that umbilical cord mesenchymal stem cells cultured in vitro can express the surface markers of neurons and glial cells under induction and exhibit replacing and repairing effects for nervous system disease.OBJECTIVE：To study the therapeutic effect and safety of transplantation of human umbilical cord blood mononuclear cells in treatment of infantile spastic cerebral palsy.METHODS：Umbilical cord blood was harvested from full term deliveries scheduled for cesarean section and anti-coagulated with heparin.Umbilical cord blood mononuclear cells were isolated by lymphocyte separation medium,purified by Nash differential adhesion,and then injected into subarachnoid cavity of 30 infantile patients with spastic cerebral palsy.RESULTS AND CONCLUSION：Compared with prior to treatment,the muscle spastic and joint range of motion significantly improved after treatment（P 0.05）.There were no adverse events and complications after transplantation and during follow-up period.

关 键 词：脐血间充质干细胞 脑性瘫痪 肌张力 关节活动度 疗效 细胞移植 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]