肾上腺髓质素对大鼠高肺血流性肺血管结构重构的干预作用  

Effect of Adrenomedullin on Pulmonary Vascular Structural Remodeling Induced by High Pulmonary Blood Flow in Rats

作　　者：高扬[1] 齐建光[1] 李晓惠[2] 庞璐璐[1] 金红芳[1] 杜军保[1] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]首都儿科研究所附属儿童医院内科,北京100020

出　　处：《实用儿科临床杂志》2012年第22期1751-1753,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30973226)

摘　　要：目的探讨肾上腺髓质素(ADM)对高肺血流性肺动脉高压大鼠肺血管结构重构的干预作用。方法 6周龄雄性SD大鼠21只,随机分为3组:对照组、分流组和分流+ADM组,每组大鼠各7只。对分流组和分流+ADM组大鼠行腹主动脉-下腔静脉分流术。对照组大鼠仅开腹暴露腹主动脉及下腔静脉,不做分流手术。8周后,对分流+ADM组大鼠,皮下埋置微量渗透泵,持续予ADM(1.5μg.kg-1.h-1)。2周后,采用右心导管法测定各组大鼠肺动脉压力,称重,并测定右心室/(左心室+室间隔)比值[RV/(LV+SP)],观察其肺动脉显微和超微结构的变化。结果与对照组比较,分流组大鼠肺动脉收缩压、肺动脉舒张压和肺动脉平均压均明显升高(Pa<0.01),RV/(LV+SP)比值明显增加(P<0.01),肺小血管肌化程度及中、小型肺动脉相对中膜厚度和相对中膜面积均明显增加(Pa<0.01),肺动脉超微结构发生明显改变,内皮细胞肿胀,内弹力层粗细不均甚至断裂,平滑肌细胞肥大,不同程度向合成表型转化。分流+ADM组与分流组比较,肺动脉压力和RV/(LV+SP)明显降低(Pa<0.01),肺动脉显微和超微结构改变明显缓解。结论通过微量渗透泵持续予ADM,能缓解大鼠高肺血流性肺动脉高压和肺血管结构重构的形成。Objective To explore the effect of adrenomedullin （ADM） on the pulmonary vascular structural remodeling in rats with pul- monary hypertension induced by high pulmonary blood flow. Methods Twenty - one male 6 - week - old SD rats were randomly divided into control group （ n = 7 ）, shunt group （ n = 7 ） and shunt with ADM group （ n = 7 ）. Aortocaval shunting was produced in rats of shunt group and shunt with ADM group. In control group, the inferior vena cava and abdominal aorta were only exposed, but shunting procedure was not per- formed. After 8 weeks, ADM （ 1.5 μg·kg^-1·h^-1 ） was subcutaneously administered into rats of shunt with ADM group by mini - osmotic pump for 2 weeks. Pulmonary artery pressures of each rat were evaluated by using a right cardiac catheterization procedure. The ratio of right ventricular mass to left ventricular plus septal mass [ RV/（LV ＋ SP） ] was calculated after weighting. The pulmonary artery micro- and ultra - rnorphologic changes of rats were observed. Results Compared with the rats of control group,pulmonary artery systolic pressure,pu- lmonary artery diastolic pressure,pulmonary artery mean pressure and RV/（ LV ＋ SP） in rats of shunt group were significantly increased （ P 〈 0.01 ） , the muscularization of small pulmonary vessels, relative medial thickness and relative medial area of the pulmonary arteries were also significantly increased （P 〈 0.01 ）. Ultrastructural changes, including swelling of endothelial cells, irregularity of inner elastic lamina, and hypertrophy and the increased number of synthetic phenotype of smooth muscle cells, were found in pulmonary arteries of shunting rats. Pulmonary artery pressure and RV/（ LV ＋ SP） in rats of shunt with ADM group were significantly decreased compared with those of shunt group （P 〈 0.01 ） ,with the alleviation of pulmonary artery micro - and ultra - morphologic changes. Conclusions ADM subcutaneously administered by mimi - osmotic pump alleviated the development of pulmo

关 键 词：肺动脉高压 高肺血流 肾上腺髓质素 血管 

分 类 号：R965[医药卫生—药理学]