Dys-psychological Stress Effect on Expressions of P53 and NFκBp65 in Human Ovarian Carcinoma In Vivo  

作　　者：Li-qun Yu Guo-lan Gao Fun-jun Liu Qiong-jing Zeng 

机构地区：[1]Department of Gynecology,Aviation General Hospital,Beijing 200022,Chine [2]Department of Gynecology and Obstetrics,First Affiliated Hospital of Nanchang University,Nanchang 330006,China

出　　处：《Chinese Journal of Cancer Research》2012年第3期245-248,共4页中国癌症研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.30860301)

摘　　要：Objective： To investigate the dys-psychological stress effect on the growth of subcutaneous xenotransplanted tumor in nude mice bearing human epithelium ovarian carcinoma, and the influence on P53 and NFκBp65 expressions. Methods： The subcutaneous tumor xenografts were established by implanting human epithelium ovarian carcinoma tissues into nude mice and the dys-psychological stress model was established with restraint. The mice were randomized into the following four treatment groups with each group six mice respectively： tumor group （group A）, normal saline intraperitoneal injection; tumor with stress group （group B）, normal saline intraperitoneal injection; tumor therapy group （group C）, cisplatin intraperitoneal injection; and tumor therapy with stress group （group D）, cisplatin intraperitoneal injection. The expressions of P53 and NFκBp65 in tumor tissues were determined by Western blotting. Results： The expressions of P53 and NFκBp65 in each restraint group were enhanced compared with the control groups （P0.05）. Conclusion： The dys-psychological stress may induce the high expressions of P53 and NFκBp65 proteins and further promote tumor growth.Objective： To investigate the dys-psychological stress effect on the growth of subcutaneous xenotransplanted tumor in nude mice bearing human epithelium ovarian carcinoma, and the influence on P53 and NFκBp65 expressions. Methods： The subcutaneous tumor xenografts were established by implanting human epithelium ovarian carcinoma tissues into nude mice and the dys-psychological stress model was established with restraint. The mice were randomized into the following four treatment groups with each group six mice respectively： tumor group （group A）, normal saline intraperitoneal injection; tumor with stress group （group B）, normal saline intraperitoneal injection; tumor therapy group （group C）, cisplatin intraperitoneal injection; and tumor therapy with stress group （group D）, cisplatin intraperitoneal injection. The expressions of P53 and NFκBp65 in tumor tissues were determined by Western blotting. Results： The expressions of P53 and NFκBp65 in each restraint group were enhanced compared with the control groups （P0.05）. Conclusion： The dys-psychological stress may induce the high expressions of P53 and NFκBp65 proteins and further promote tumor growth.

关 键 词：Constraint stress ONCOPROTEIN Ovarian cancer animal model 

分 类 号：Q2[生物学—细胞生物学] T-1[一般工业技术]