超声波-缺氧/好氧污泥消化反应器中氨氧化细菌群落结构的演变  

Succession of ammonia-oxidizing bacterial community in ultrasonic-aerobic/anoxic digestion reactor

作　　者：郑莉[1] 孙水裕[1] 许燕滨[1] 潘南全 刘宝建 叶运弟[1] 占星星[1] 

机构地区：[1]广东工业大学环境科学与工程学院,广州510006 [2]广州市沥滘污水处理厂,广州510290

出　　处：《环境工程学报》2012年第12期4462-4468,共7页Chinese Journal of Environmental Engineering

基　　金：广东省教育部产学研合作专项(2008B0905200253)

摘　　要：为了揭示超声波-缺氧/好氧污泥消化反应器中氨氧化细菌群落结构多样性的演变过程,采用变性梯度凝胶技术(PCR-DGGE)研究不同运行时期氨氧化细菌群落结构的变化。DGGE分析表明,反应器中氨氧化细菌群落比较丰富。在反应器运行的不同时期,氨氧化细菌的群落结构发生了一定的变化,反映了种群的动态演变。UPGMA聚类分析将DGGE图谱分为3大类群并对应于各自的运行时期。测序结果表明,反应器中的优势种群属于变形菌门(Proteobacteria),包括α-proteobacteria,β-proteobacteria,γ-proteobacteria和ε-proteobacteria4个纲,其中β-proteobacteria占45%,γ-proteobacteria占40%。在始终保持明显优势地位的种属中,5株为反硝化细菌,它们对提高反应器脱氮效率具有重要作用。In order to explore the succession of ammonia-oxidizing bacterial community diversity in ultra- sonic-anoxic/aerobic digestion reactor, PCR-DGGE technique was used to investigate the variation of ammonia- oxidizing bacterial community structure at different operational time points. DGGE analysis indicated that the am- monia-oxidizing bacterial diversity was rich. The ammonia-oxidizing bacterial community structure changed under different operation conditions, which reflecting the dynamic succession of ammonia-oxidizing bacterial communi- ty. Cluster analysis of DGGE using UPGMA （unweighted pairgroup method using arithmetic average） revealed that there were three clusters, which corresponded to three different operation periods, respectively. The sequen- cing results indicated that ammonia-oxidizing bacteria species in reactor are mainly composed of Proteobacteria, including α-proteobacteria, β-proteobacteria, γ-proteobacteria and ε-proteobacteria. However, the proportions of β-proteobacteria and γ-proteobacteria are 45% and 40% , respectively. Among the predominant species through the operation, 5 of them are denitrifiers which play an important role in improving the efficiency of nitrogen re- moval.

关 键 词：超声波预处理 缺氧 好氧消化 微生物多样性 PCR—DGGE 氨氧化细菌 

分 类 号：TU991[建筑科学—市政工程]