改良Genome shuffling技术选育埃博霉素B高产菌株  

Screening and breeding of high epothilone B-producing strains by improvement of genome shuffling

作　　者：龚国利[1] 陈松[1] 李慧[2] 曾桥[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021 [2]陕西科技大学资源与环境学院,陕西西安710021

出　　处：《中国酿造》2012年第11期42-46,共5页China Brewing

基　　金：国家自然科学基金(No.20906058);陕西省自然科学基金(No.2010JQ4020);陕西省教育厅自然科学研究专项(No.12JK1023);陕西省科技厅社会发展攻关项目(No.2010K01-200);陕西科技大学学术骨干培育项目(No.XSG2010009)

摘　　要：目的:用改良genome shuffling技术选育埃博霉素高产菌株。方法:本研究对genome shuffling技术进行了改良,具体是在递归原生质体融合过程中对原生质体进行了紫外诱变;通过UV和DES复合诱变方法获得了4株出发菌株,并用改良genome shuffling技术选育埃博霉素B高产菌株。结果:通过三轮基因组重组成功选育到了2株遗传稳定的高产埃博霉素B重组菌株,其中一株重组菌株So073-45的埃博霉素B产量达到了42.5mg/L。结论:该研究证明改良后的基因组重组技术不仅能有效提高埃博霉素B的产量,而且比传统的基因组重组技术更为高效。Objective To screen and breed high epothilone B producing strains by improvement of genome shuffling. Methods This study improved the genome shuffling techniques, Protoplast was mutated with UV irradiation in the fusion process; The study using UV and DES compound mutation method screened out four start strains, and high epothilone B-producing strains were obtained by improvement of genome shuffling. Results Two hereditarily stable strains with high epothilone B production were obtained by three cycles of genome shuffling. Among them, strain So 073-45 epothilone B production reached 42.5mg/L. Conclusion The study proves that improvement ofgenome shuffling can not only improve the production of epothilone B, and than traditional genome shuffling more efficient.

关 键 词：埃博霉素B 基因组重组 纤维堆囊菌 原生质体诱变 

分 类 号：Q93-331[生物学—微生物学]