液相色谱-串联质谱法快速检测番茄制品中苏丹红  

Study on rapid determination of sudan red in tomato products by LC-MS/MS method

作　　者：张云 李今中 郑敬峰 李耀平[2] 吕园园 张信仁 李晓捷 

机构地区：[1]三明出入境检验检疫局,三明365000 [2]福建出入境检验检疫局技术中心,福州350001

出　　处：《分析试验室》2012年第12期78-81,共4页Chinese Journal of Analysis Laboratory

基　　金：福建检验检疫局科技项目(FK2011-48)资助

摘　　要：采用HPLC/MS-MS鉴定和检测番茄制品中苏丹红染料Ⅰ～Ⅳ。番茄制品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ,经正己烷加乙酸溶液提取,氧化铝柱净化,再用二氯甲烷洗脱苏丹红,经浓缩至干,流动相定容,液相色谱串联质谱测定,外标法定量。将已用HPLC法测定过的10个可疑样品,用该方法进行检测,结果有4个检了苏丹红Ⅰ。采用本方法检测苏丹红Ⅰ～Ⅳ,检测限均可达到1.0μg/kg,在添加浓度1～100μg/kg之间线性关系良好,相关系数(r)均>0.9990,对于1、5、10μg/kg水平添加回收率在72.3%～103%之间,相对标准偏差在2.5%～9.8%之间。To develop a method for simultaneous characterization and determination of 4 kinds of sudan dyes in the tomato products by high performance liquid chromatography-tandem mass spectrometry（HPLC/MS-MS）.The sudanⅠ,Ⅱ,Ⅲ,Ⅳ in tomato products were extracted by hexane and acetic acid aqueous solution,purified by an alumina column,and then sudan dyes were eluted by methylene chloride,concentrated to dry,and dissolved by the mobile phase in a constant volume,determined by liquid chromatography tandem mass spectrometry and quantified with the external standard method.10 suspicious samples determined by HPLC were detected by using this method,and sudan I was found in 4 tested samples.This method was applied to the determination of sudanⅠ~Ⅳ,the detection limits were 1.0μg/kg,and the linear ranges were 1～100 μg/kg with correlation coefficients larger than 0.9990.The recoveries were 72.3%～103% at the spiked levels of 1,5,10 μg/kg and the relative standard deviations（RSDs） were 2.5%～9.8%.The method has good sensitivity and precision.

关 键 词：番茄制品 苏丹红 HPLC MS-MS 

分 类 号：O657.63[理学—分析化学]