缺氧对人肝癌细胞MHCC-97L迁移的影响  

Hypoxia induces the migration of MHCC-97L hepatic carcinoma cells

作　　者：张万秋[1] 朱一超[2] 

机构地区：[1]泰州职业技术学院,江苏泰州225300 [2]南京医科大学生理学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2012年第11期1522-1526,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金青年基金(81101999)

摘　　要：目的:研究缺氧对人肝癌细胞MHCC-97L迁移的影响,并初步揭示其分子机制。方法:建立划痕实验检测缺氧条件下人肝癌细胞MHCC-97L迁移能力变化的可靠模型,研究Wnt5a对MHCC-97L细胞迁移的调控。结果:缺氧使MHCC-97L细胞缺氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)和Wnt5a mRNA表达上调,并可促进MHCC-97L细胞迁移能力显著增强,干扰HIF-1α表达的MHCC-97L细胞在缺氧条件下Wnt5a mRNA表达显著降低,干扰Wnt5a表达的MHCC-97L细胞在缺氧环境中的迁移能力则明显降低。结论:在缺氧条件下MHCC-97L细胞迁移能力可发生明显改变,HIF-1α和Wnt5a调控其迁移过程。这些结果为深入阐明肝癌细胞转移和侵袭分子调控机制提供了新的实验线索。Objective：To elucidate the function of hypoxia,and investigate the underlying mechanisms whereby hepatic carcinoma cell migration is regulated.Methods：Under hypoxia microenvironment,the migration of MHCC-97L hepatic carcinoma cells were detected using wound healing assay.Then we analyzed the migration of MHCC-97L cells after blocking Wnt5a expression using siRNA.Results：The mRNA transcription of HIF-1α and Wnt5a in MHCC-97L cells were upregulated under hypoxia microenvironment.HIF-1α siRNA suppressed the mRNA transcription of Wnt5a in MHCC-97L cells under hypoxia microenvironment.Wnt5a siRNA were capable of retarding hypoxia-induced MHCC-97L cell migration.Conclusion：It is demonstrated that hypoxia promotes MHCC-97L cell migration via HIF-1α and Wnt5a signaling.These findings could provide a rationale for designing novel therapy based on inhibition of hepatic carcinoma metastasis.

关 键 词：缺氧 MHCC-97L 肝癌细胞 迁移 WNT5A 

分 类 号：R73-37[医药卫生—肿瘤]