小鼠颈髓后索溶血卵磷脂注射诱导轴突脱髓鞘  

Lysolecithin-induced axonal demyelination in the dorsal funiculus of mice cervical spinal segment

作　　者：张永杰[1] 吴琪[1,2] 左一智[1] 肖娜[1] 李雷[1] 顾振[1] 

机构地区：[1]南京医科大学人体解剖学系,江苏南京210029 [2]中国人民解放军八二医院骨科,江苏淮安223001

出　　处：《南京医科大学学报（自然科学版）》2012年第11期1537-1540,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学青年基金(81100942);江苏省自然科学基金(BK2010539);江苏高校优势学科建设工程项目;南京医科大学基础医学院青年教师培养基金(09JC012);南京医科大学基础医学院优势学科项目(JX10131801075)

摘　　要：目的:注射溶血卵磷脂(lysolecithin,LPC)至小鼠颈段脊髓后索诱导轴突脱髓鞘的模型建立及评价体系。方法:手术显微镜下,使用微量注射器将0.4μl 1%LPC注射至成年小鼠第5颈髓(C5)后索。1周后处死动物,取C4～C6颈髓节段,行石蜡切片、甲苯胺蓝染色观察脱髓鞘区域,采用Amira 4.1.1软件三维重建和测量脱髓鞘区域体积,并进行电镜观察轴突脱髓鞘病变。结果:注射LPC造模,小鼠苏醒后无明显运动及感觉异常,甲苯胺蓝染色显示注射区出现体积为(1.41±0.05)×106μm3淡蓝或白色脱髓鞘病变区,电镜结果证实注射区轴突发生脱髓鞘样变。结论:小鼠颈髓后索注射LPC可稳定诱导轴突脱髓鞘。Objective：To establish an effective model and evaluation system for lysolecithin（LPC） induced demyelination in dorsal funiculus of cervical spinal segment in the adult mice.Methods：The adult mice were injected with 0.4 μl 1% LPC or normal saline into the dorsal funiculus of the 5th cervical（C5） spinal segment using glass-needle-capped syringe under the microscope.One week post surgery,mice were sacrificed and perfused,and segments of C4 to C6 were dissected and embedded in paraffin.Serial coronal sections were stained with toludine blue.The 3D cartoon of demyelinated area of total serial sections was rebuilt,and the volume of the demyelinated area was measured by Amira 4.1.1 software.The demyelinated axons were also observed by electron microscope.Results：No obvious motor and sensory abnormity were found after mice recovered from the anesthesia.Toludine blue staining result showed that there was the light blue or pale demyelination area around the injection point,and the volume of which was about（1.41 ± 0.05） × 10^6 μm^3.The demyelinated axons were confirmed by electron microscopy observation.Conclusion：Precise injection of LPC into the C5 dorsal funiculus of mice can stably induce the axonal demyelination.

关 键 词：脱髓鞘 脊髓后索 溶血卵磷脂 

分 类 号：R744.5[医药卫生—神经病学与精神病学]