一株拮抗纹枯病菌放线菌的筛选及鉴定  

Screening and identification of antagonistic actinomycete against Rhizoctonia solani

作　　者：崔宇辉[1] 王媛媛[1] 汪鹏荣[1] 高爱同[1] 曹丽凌[1] 蒋冬花[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2012年第4期441-445,共5页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省公益性应用研究计划项目(2010C32076)

摘　　要：从采自金华市各地的68份土样中分离得到了放线菌406株,以水稻纹枯病菌为指示菌,利用平皿对峙法筛选到42株具有较好拮抗作用的放线菌菌株.其中,Sh-43菌株的拮抗作用最强,抑菌带宽度为28.3mm.根据培养特征、形态特征、生理生化特征及16S rDNA序列比对分析,鉴定Sh-43菌株为吸水链霉菌(Strep-tomyces hygroscopicus).Four hundred and six actinomycetes were isolated from sixty eight soil samples which were collected from different areas of Jinhua. Forty-two strains had been proved to have antagonistic activity against Rhizocto- nia solani by confronting cultivation on PDA plates. Among them, the strain Sh-43 exhibited the strongest an- tibiosis capacity with an inhibition zone of 28.3 mm. The strain Sh-43 was identified as scopicus on the cultural, morphological, physiological, biochemical characteristics and 16S rDNA gene se- quence analysis.

关 键 词：纹枯病菌 放线菌 拮抗作用 筛选 鉴定 

分 类 号：Q93-331[生物学—微生物学]