Simulation of Reductive Dechlorination Processes in a Lab-Scale Anaerobic Biobarrier with Enriched TCP Dechlorinating Consortium  

作　　者：LI Zhiling INOUE Yasushi MIZOGUCHI Takuya SIMIZU Yohei YOSHIDA Naoko KATAYAMA Arata 

机构地区：[1]EcoTopia Science Institute, Nagoya University, Nagoya 464-8603, Japan [2]Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan [3]Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Toyohashi 441-8580, Japan

出　　处：《Transactions of Tianjin University》2012年第6期441-449,共9页天津大学学报（英文版）

基　　金：Supported by Grant-in-Aids for Scientific Research from the Ministry of Education,Culture,Sports,Science and Technology of Japan (No.23310055 and No.23658272)

摘　　要：In order to design and predict the dechlorination processes for remediating the halogenated aromatic com- pounds in the biobarrier system applied in situ, an anaerobic continuous-flow column was set up with the introduction of an enriched 2, 4, 6-trichlorophenol （TCP） reductive dechlorinating consortium. The fates of TCP and its metabo- lites were simulated according to the first-order sequential dechlorination kinetic model. The enriched TCP anaerobic dechlorinating consortium dechlorinated 100 p.mol/L TCP to 4-chlorophenol （4-CP） via 2,4-dichlorophenol （DCP） in 10 d. The consortium was predominated with the phylum of Firmicutes and Bacteroidetes, based on the PCR- denaturing gradient gel electrophoresis （DGGE） analysis. After the consortium was applied to the colunm, the ex- perimental data in the steady state were fitted by the least square method, and the first-order dechlorination kinetic constants from TCP to 2,4-DCP, from 2,4-DCP to 4-CP and from 4-CP to phenol, were 1.58 d-1, 2.23 d 1 and 0.206 d-1, respectively. According to the fitting results, the required biobarrier width for the complete remediation of TCP, 2,4- DCP and 4-CP were 126 cm, 130 cm and 689 cm, respectively. The dechlorination/degradation of 4-CP must be in- creased when the technology is applied to the real site.In order to design and predict the dechlorination processes for remediating the halogenated aromatic compounds in the biobarrier system applied in situ,an anaerobic continuous-flow column was set up with the introduction of an enriched 2,4,6-trichlorophenol(TCP) reductive dechlorinating consortium.The fates of TCP and its metabolites were simulated according to the first-order sequential dechlorination kinetic model.The enriched TCP anaerobic dechlorinating consortium dechlorinated 100 μmol/L TCP to 4-chlorophenol(4-CP) via 2,4-dichlorophenol(DCP) in 10 d.The consortium was predominated with the phylum of Firmicutes and Bacteroidetes,based on the PCRdenaturing gradient gel electrophoresis(DGGE) analysis.After the consortium was applied to the column,the experimental data in the steady state were fitted by the least square method,and the first-order dechlorination kinetic constants from TCP to 2,4-DCP,from 2,4-DCP to 4-CP and from 4-CP to phenol,were 1.58 d-1,2.23 d-1 and 0.206 d-1,respectively.According to the fitting results,the required biobarrier width for the complete remediation of TCP,2,4DCP and 4-CP were 126 cm,130 cm and 689 cm,respectively.The dechlorination/degradation of 4-CP must be increased when the technology is applied to the real site.

关 键 词：biobarrier 2 4 6-TCP anaerobic dechlorination first-order kinetic model SIMULATION 

分 类 号：X132[环境科学与工程—环境科学] Q141[生物学—生态学]