MicroRNA-21与乳腺癌细胞MDA-MB-231放射敏感性之间关系的研究  

Study on the relationship between microRNA-21 and radiation sensitivity of breast cancer cell line MDA-MB-231

作　　者：狄英波[1] 孙颖[2] 张桂英[2] 刘姗姗[2] 薛茗方[2] 王宝贵[2] 赵刚[3] 

机构地区：[1]吉林省妇幼保健院,吉林长春130061 [2]吉林大学公共卫生学院营养与食品卫生学教研室 [3]吉林大学公共卫生学院卫生部放射生物学重点教研室

出　　处：《中国妇幼保健》2012年第34期5571-5573,共3页Maternal and Child Health Care of China

基　　金：吉林省科学技术厅科技支撑计划〔20090932〕;吉林省科学技术厅科技引导计划〔201105020〕

摘　　要：目的:研究MicroRNA-21与乳腺癌细胞MDA-MB-231放射敏感性之间关系及其表达规律。方法:①对乳腺癌细胞MDA-MB-231分别转染miR-21 mimics、miR-21 mimics Scramble(阴性对照)、miR-21 inhibitor、miR-21 inhibitorScramble(阴性对照),转染后进行集落形成实验,通过存活分数(Surviving Fraction SF)的变化检测其辐射敏感性是否发生变化;②时程实验:3Gy X-Ray照射后,分别于0、4、8、12、24 h后,实时荧光定量PCR检测miR-21的表达;③量效实验:0、1、2、4、6Gy X-Ray分别照射细胞24 h后,实时荧光定量PCR检测miR-21的表达。结果:MDA-MB-231细胞转染miR-21mimics后,SF明显升高,与正常对照组比较有显著性差异(P<0.05);MDA-MB-231细胞转染miR-21 inhibitor后,SF明显降低,与正常对照组比较有显著性差异(P<0.05);3GyX射线照射乳腺癌细胞株MDA-MB-231后,miR-21表达量上调,与对照组比较差异具有显著性(P<0.05),并在12 h时达到峰值,24 h时恢复到照前水平;miR-21的表达量随着照射剂量的增加而发生变化,2Gy时达到峰值,照射剂量进一步加大,miR-21表达量开始下调,与对照组比较具有显著性差异(P<0.05),6Gy时miR-21表达量明显低于未照射组。结论:miR-21对于增加乳腺癌细胞株MDA-MB-231的辐射抗性具有一定作用。Objective： To research the relationship between microRNA - 21 and radiation sensitivity of breast cancer cell line MDA -MB -231 and the expression regularity. Methods： Breast cancer cell line MDA - MB -231 was transfected by miR -21 mimics, miR -21 mimics Scramble （negative control group ）, miR -21 inhibitor, and miR -21 inhibitor Scramble （negative control group ）, respectively, then colony -forming assay was performed, the change of surviving fraction （SF） was used to detect whether radiation sensitivity changed, there was statistically significant difference. Time -course experiment： after radiating with 3Gy X -ray, real -time fluorescent quantitative polymerase chain reaction （PCR） was used to detect the expression of miR- 21 at 0, 4, 8, 12, and 24 hours, there was statistically significant difference. Dose - effect experiment ： after radiating the cells with 0, 1, 2, 4, 6Gy X ray for 24 hours, the expression of miR-21 was detect by real -time fluorescent quantitative PCR. Results： After transfeeting MDA -MB -231 cells with miR -21 mimics, SF increased statistically significantly, compared with normal control group, there was statistically significant difference （ P 〈 0. 05 ） ; after transfecting MDA- MB- 231 cells with miR- 21 inhibitor, SF decreased statistically significantly, compared with normal control group, there was statistically significant difference （P 〈 0. 05 ） ; after radiating breast cancer cell line MDA - MB -231 with 3Gy X ray, the expres- sion level of miR -21 was up - regulated, compared with normal control group, there was statistically significant difference （P 〈 O. 05 ）, and the expression level of miR -21 peaked at 12 hours, then it returned to the level before radiation; the expression level of miR -21 changed with the dose of radiation, which peaked at the dose of 2 Gy, while the expression level of miR -21 was down -regulated with further increase of dose of radiation, compared with normal control group, there was statistically significant

关 键 词：放射敏感性 MICRORNA-21 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]