鹅MAPK14基因部分序列克隆及填饲对其mRNA丰度的影响  

Cloning of Partial Sequence of MAPK14 Gene of Goose and the Effect of Overfeeding on Its mRNA Expression Level

作　　者：何桦[1] 王浩瀚[1] 刘贺贺[1] 潘志雄[1] 王继文[1] 

机构地区：[1]四川农业大学动物遗传育种研究所,四川成都611130

出　　处：《中国畜牧杂志》2012年第23期6-10,共5页Chinese Journal of Animal Science

基　　金：国家现代农业水禽产业技术体系(CARS-43-6)

摘　　要：为研究填饲对鹅丝裂原活化蛋白激酶14(MAPK14)mRNA表达水平的影响,本实验克隆了鹅MAPK14部分编码区序列,并用荧光定量PCR方法检测了填饲对四川白鹅和朗德鹅肝组织MAPK14基因表达量的影响。结果表明:获得鹅MAPK14基因cDNA 951 bp序列,可编码316个氨基酸残基。经分析,鹅MAPK14部分核苷酸序列与原鸡MAPK14基因的同源性为94.8%,对应的氨基酸同源性为99.7%;荧光定量PCR检测发现,无论在对照组还是填饲组,四川白鹅的MAPK14表达量均高于朗德鹅,填饲能显著上调MAPK14 mRNA在2个鹅品种中的表达丰度。结果提示,填饲能够引起鹅肝组织中MAPK14表达丰度显著增加,且该影响存在显著的品种差异。To understand the effect of overfeeding on Mitogen-activated Protein Kinases 14（MAPK14）mRNA level, the goose MAPK14 gene partial sequence was cloned by RT-PCR in this study, and the goose MAPK14 gene expression in liver tissue was detected by Q-PCR. The result indicated that the obtained MAPK14 gene sequence was 951 bp coding 316 amino acid residues, the nucleotide sequence and amino acid sequence share 94.8%, 99.7% identity with the gallus MAPK14; The Q-PCR detection found that overfeeding caused the MAPK14 mRNA expression increased significantly in both two breeds, the mRNA level of MA PK14 in liver of Sichuan White goose was significantly higher than that of Landes goose between the control group and the overfeeding group. It was concluded that overfeeding induced the significant increase of MAPK14 mRNA level in goose liver tissues, and the effect of overfeeding was significant difference between the two breeds.

关 键 词：填饲 MAPK14 克隆 MRNA表达 鹅 

分 类 号：S835.2[农业科学—畜牧学]