盐碱土壤PAHs降解真菌筛选及降解特性研究  

Isolation and Biodegradation Characteristics of Degrading Fungus Isolated from Salt-soda Soil

作　　者：宋立超[1,2] 钮旭光[1] 李培军[2] 刘宛[2] 肖亦农[1] 张玉龙[1] 

机构地区：[1]沈阳农业大学土地与环境学院,辽宁沈阳110866 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《土壤通报》2012年第6期1397-1400,共4页Chinese Journal of Soil Science

基　　金：中国科学院知识创新工程重大项目(京津塘区域环境污染调控技术与示范)子课题"土壤污染控制修复技术及其应用示范(kzcxl-yw-06-03)";沈阳农业大学青年教师科研基金(20111006)资助

摘　　要：从天津大港油田PAHs污染的盐碱土壤中分离到1株以菲、芘为唯一碳源的真菌TJF1,经形态观察为青霉属（Penicillium）。菌株TJF1在含菲、芘浓度分别为25、50和75 mg L-1的液体无机盐培养基中培养15 d降解率为75.7%和50.1%、41.3%和27.9%、23.9%和12.5%。菌株TJF1对菲、芘的降解具有较广泛的pH和盐度范围,在菲、芘总浓度100 mg L-1,pH5.3~9.5,盐度0%~10%的液体条件下均可生长及有较高的降解效果,为了提高其对菲、芘的降解效率,在底物中添加一定量的葡萄糖,结果表明：葡萄糖对菲、芘降解率影响呈抛物线状,葡萄糖浓度在0.8%时,TJF1对菲、芘的降解率达到63.8%和57.2%。The predominant indigenous microbial population and the strain TJF1 PAHs-degradation were developed by selective enrichment culture from oil-contaminated saline soil in Dagang Oilfield. Morphology analysis revealed that strain TJF1 belonged to Penicillium. When PAHs initial concentration was 25 mg L-l, 500 mg L-1 and 75 mg L-l, the degradation rates of phenanthrene and pyrene by TJF1 were separately 75.7% and 50.1%, 41.3% and 27.9%, 23.9% and 12.5%. Phenanthrene and pyrene could be biodegraded at different pH values ranging from 5.3 to 9.5 and different salinity ranging from 0% to 5% in the medium contains 100 mg L-1 phenanthrene and pyrene. Our results indicated that more than 63.8% of phenanthrene and 53.2% pyrene were degraded in the medium containing 0.8% glucose.

关 键 词：盐碱化 多环芳烃 青霉菌 降解特性 

分 类 号：X172[环境科学与工程—环境科学]