雌激素对大鼠血管钙化消退的影响  

Effect of estrogen on regression of vascular calcification in rats

作　　者：孙文学[1] 刘毅[1] 阮吉[2] 徐玉兰[1] 

机构地区：[1]温州医学院附属第一医院肾内科,325000 [2]浙江省台州市立医院肾内科

出　　处：《中华肾脏病杂志》2012年第11期879-882,共4页Chinese Journal of Nephrology

摘　　要：目的研究雌激素对尼古丁和维生素D3（VDN）诱发的大鼠血管钙化消退过程的影响。方法96只SD雌性大鼠随机分为对照组（n=24）和钙化组（n=72）。首先制备VDN大鼠钙化模型，第1天维生素D3300000U／kg肌肉注射，尼古丁（25mg／kg溶于花生油）灌胃，9h后重复灌胃1次。对照组8只大鼠予生理盐水0．2ml肌注及单纯花生油灌胃。实验第4周末将血管钙化的大鼠（n=64）随机分为单纯钙化组（VDN，n=16）、钙化＋假手术组（VDN＋SHAM，n=16）、钙化＋去势组（VDN＋OVX，n=16）、钙化＋去势＋补充雌激素组（VDN＋OVX＋E，n=16）。去势组为双侧卵巢切除，假手术组为切开腹腔，暴露双侧卵巢后关闭腹腔（不切除卵巢），去势＋补充雌激素组为去势后皮下注射17β-雌二醇50μg·kg^-1·d^-1。放射免疫法测定血浆雌激素水平。在第4、8、12周末各组分别处死8只老鼠，观察各组血管钙化变化的情况。结果成功制备了血管钙化的大鼠模型，第4周末，钙化组血管钙化明显，动脉中层可见大量钙盐沉积，血管钙含量显著高于对照组（P〈0．01）。第8周、12周末各钙化组大鼠的血管钙含量均较第4周减少（P〈0．01），钙化＋去势＋补充雌激素组最为显著，且各组之间差异亦有统计学意义（P〈0．01），病理变化类同。结论尼古丁和维生素D3诱发的大鼠血管钙化是可以消退的，在雌激素的作用下，血管钙化消退更加明显。Objective To investigate the effect of estrogen on regression of vascular calcification in rats induced by vitamin D3 plus nicotine. Methods Ninety-six female SD rats were divided randomly into control group （n=24） and calcification group （n=72）. Vascular calcification of 72 rats was induced by vitamin D3 and nicotine （VDN）. On the day 1, the VDN group rats were injected with vitamin D3（300 000 U/kg, i.m）, and were intragastric administrated with nicotine （25 mg/kg）, after 9 hours, another dosage of nicotine was given again. After 4 weeks, the VDN group rats were subdivided randomly into 4 groups： VDN group（n=16）, Sham operation group （n=16）, ovariotomy group （n=16）, estrogen group（after ovariotomy, 1713 -estrogen was subcutaneously injected, 50 μg·kg^-1·d^-1, n= 16）. Results After 4 weeks, the VDN group showed obvious vascular calcification, and calcium content of the vessel wail was significantly higher than that of control group （P〈0.01）. Extensive calcification was witnessed on the aortic tunica media of the VDN group. After 12 and 8 weeks, the calcium content of the vessel wall in each subdivided groups was significantly lower than that at 4 weeks point（P〈0.01）, and the lowest calcinm content was in estrogen group, meanwhile the reduction of previously accumulated arterial calcium precipitate in each group was different. Conclusions It is a reversible process that vascular calcification induced by vitamin D plus nicotine in rats. Estrogen can promote the regression of vascular calcification.

关 键 词：钙质沉着症 模型 动物 雌激素 消退 

分 类 号：R363[医药卫生—病理学]