核转录因子κBp65在肝炎后肝癌中的表达  

Expression of NF-κBp65 in hepatocellular carcinoma following hepatitis

作　　者：张颖[1] 赵鑫[2] 姜秀娟[1] 关胜江[1] 高庆军[2] 

机构地区：[1]河北医科大学基础课教学部,河北石家庄0500001 [2]河北医科大学第三医院肝胆外科,河北石家庄0500001

出　　处：《军医进修学院学报》2012年第12期1287-1289,1292,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：河北省卫生厅指导性课题(20110278)

摘　　要：目的探讨肝癌组织、癌旁肝炎组织中核转录因子κBp65(nuclearfactorκBp65,NF-κBp65)的定量表达及与细胞DNA含量、细胞增殖周期的关系。方法对50例肝细胞性肝癌组织、癌旁肝炎组织和10例非癌旁来源的正常肝组织及非癌旁来源的病毒性肝炎组织,采用流式细胞术检测NF-κBp65含量、细胞DNA含量和增殖指数。结果 NF-κBp65在正常组织、非癌旁来源的肝炎组织、癌旁肝炎组织和肝癌中荧光指数(FI)值分别为1.00±0.19,1.28±0.21,1.32±0.15和1.66±0.23,肝癌NF-κBp65含量高于癌旁肝炎组织(P<0.05)。正常组织、非癌旁来源的肝炎组织、癌旁肝炎组织和肝癌的DNA指数(DI)值分别为1.02±0.17、1.04±0.11,1.09±0.18和1.40±0.22,肝癌异倍体率达82.00%,癌组的DNA含量和异倍体率均高于其他组(P<0.05)。正常组织、非癌旁来源的肝炎组织、癌旁肝炎组织和肝癌的增殖指数(PI)值分别11.00±2.79、11.95±2.16,12.81±2.87和24.28±4.19,癌组PI值显著高于其他组(P<0.05)。结论 NF-κBp65过量表达、DNA含量增加和增殖指数升高与肝炎后肝癌的发生发展密切相关。Objective To study the quantitative expression of NF- κBp65 protein in hepatocellular carcinoma （HCC） and its adjacent hepatitis tissue and its relation with DNA content and cell proliferation cycle. Methods NF- κBp65 expression and DNA content in HCC and its adjacent hepatitis tissue from 50 HCC patients and 10 controls and cell proliferation index （PI） were detected by flow cytometry. Results The fluorescent index （FI） of NF- κBp65 was 1.00 ± 0.19, 1.28 ± 0.21, 1.32 ± 0.15 and 1.66 ± 0.23, respectively, in normal hepatic tissue, non-HCC hepatitis tissue, adjacent hepatitis tissue and HCC tissue. The NF-κBp65 expression level was higher in HCC tissue than in its adjacent tissue （P〈0.05）. The DNA index （DI） was 1.02 ± 0.17, 1.04 ± 0.11, 1.09 ± 0.18 and 1.40 ± 0.22, respectively, in normal hepatic tissue, non-HCC hepatitis tissue, adjacent hepatitis tissue and HCC tissue, with the heteroploid in HCC reached 82.00%. The DNA level and heteroploid rate were higher in HCC tissue than in other tissues （P〈0.05）. The cell PI was 11.00 ± 2.79, 11.95 ± 2.16, 12.81 ± 2.87 and 24.28 ± 4.19, respectively, in normal hepatic tissue, non-HCC hepatitis tissue, adjacent hepatitis tissue and HCC tissue, and was significantly higher in HCC tissue than in other tissues （P〈0.05）. Conclusion Over-expression of NF-κBp65, high DNA content and elevated cell PI are closely related with HCC following hepatitis.

关 键 词：肝炎 肝癌 核转录因子κBp65 DNA 

分 类 号：R735.7[医药卫生—肿瘤]