HPLC测定6种蜂蜜中5-羟甲基糠醛的含量  

Determination of 5-HMF in Six Different Kinds of Honeys by HPLC

作　　者：龙光明[1] 裘璐[1] 马丽[1] 郝小燕[1] 

机构地区：[1]贵阳医学院药学院,贵阳550004

出　　处：《数理医药学杂志》2012年第6期643-645,共3页Journal of Mathematical Medicine

基　　金：贵州省科技厅工业攻关项目(黔科合GY字[2009]3053)

摘　　要：目的:建立蜂蜜中5-羟甲基糠醛(5-HMF)的含量测定方法。方法:利用高效液相色谱法测定6种蜂蜜中5-HMF的含量。结果:5-HMF在48.64～291.84ng范围内呈良好的线性关系,r=0.9999;平均回收率为100.02%,RSD为1.69%(n=6);6种蜂蜜中5-HMF的平均含量在0.0328～0.0398mg.g-1。结论:所建立的方法简便,重复性好,可用于检测蜂蜜中5-HMF的含量。Objective： To establish a HPLC method for the determination of 5-HMF in different types of honeys. Methods： HPLC method was applied to the determination of 5- HMF in honeys. Results： 5-HMF had a good linearity within the range of 48. 64-291.84 ng, r=0. 9999, The average recovery was 100.02%, RSD=1. 69%（n=6） ; The average contents of six types of honeys were 0. 0328 - 0. 0398 mg · g-1. Conclu- sions： The method is simple and repeatable , which can be used to determine the content of 5-HMF in hon- eys.

关 键 词：蜂蜜 5-羟甲基糠醛 HPLC 

分 类 号：R971[医药卫生—药品]