血浆早老蛋白-1对溃疡性结肠炎及克罗恩病的诊断价值  

Research on the Value of Plasma Presenilin-1 in the Diagnosis of Ulcerative Colitis and Crohn＇sDisease

作　　者：沈骏[1] 乔宇琪[1] 冉志华[1] 童锦禄[1] 朱明明[1] 王天蓉[1] 黄美兰[1] 

机构地区：[1]上海交通大学医学院附属仁济医院,上海市消化疾病研究所,上海200001

出　　处：《医学临床研究》2012年第11期2054-2056,共3页Journal of Clinical Research

基　　金：国家自然科学基金（81000161）,上海交通大学医学院科技基金（YZ1036）,上海交通大学医学院附属仁济医院学科建设（RJ4101305）

摘　　要：【目的】研究炎症性肠病患者血浆早老蛋白-1（Presenilin-1）的表达和对溃疡性结肠炎及克罗恩病的鉴别诊断价值。【方法】应用酶联免疫吸附试验（ELISA）分析克罗恩病患者、溃疡性结肠炎患者和正常对照者血浆中Presenilin1的表达水平。通过受试者工作特征（receiver-operatingcharacteristic，ROC）曲线观察血浆Presenilin-1水平对溃疡性结肠炎患者和克罗恩病患者的鉴别诊断价值。【结果】溃疡性结肠炎患者血浆Presenilin-1水平显著高于正常对照者（Pd0．01）和克罗恩病患者（P=0．0053），同时血浆对Presenilin-1水平对克罗恩病患者和溃疡性结肠炎患者具有显著的鉴别价值（AUC=0．7657，P〈0．01），但是溃疡性结肠炎患者血浆Presenilin-1与内镜疾病活动性无显著相关性（Spearman r=0．1224，P=0．4022）。【结论】溃疡性结肠炎患者血浆Presenilin-1表达水平增高，可能对鉴别诊断克罗恩病有一定价值。[Objective] To explorethe plasma expression of Presenilin 1 in patients with inflammatory bowel disease and its value in the differenti- al diagnosis of ulcerative colitis and Crohn＇s disease. [Methods] Enzyme-linked immunosorbent assay（ELISA） was applied to analyze plasma level of Presenilin-1 in patients with Crohn＇s disease, ulcerative colitis and healthy controls. Receiver operating characteristic（ROC） curve was used to observe differential diagnostic val- ue of plasma level of Presenilin-1 in patients with ulcerative colitis and Crohn＇s disease. GraphPad Prism 5.0 was used for the data processing. [Results] Plasma level of Presenilin-1 in patients with ulcerative colitis was significantly higher than that in normal controls （ P〈0.01） and patients with Crohn＇s disease（ P =0. 0053）. Plasma level of Presenilin-1 had significant diagnostic value in patients with ulcerative colitis and Crohn＇s dis- ease （AUC=0. 7657, P d0.01）. However, there was no significant correlation between plasma level of Pre- senilin-1 and endoscopic disease activity in patients with ulcerative colitis（Spearman r=0. 1224, P =0. 4022）. [Conclusion] Plasma level of Presenilin-1 in patients with ulcerative colitis increased, which may contribute to the differential diagnosis of ulcerative colitis and Crohn＇s disease.

关 键 词：结肠炎 溃疡性 血液 C rohn病 血液 

分 类 号：R574[医药卫生—消化系统]