黄芪对肝星状细胞基质分解素-1及其抑制因子基因表达的调节  

Regulation of Astragalus on gene expression of stromelysin-1 and tissue inhibitor of metalloprotein- ase-1 in HSC-T6 cells

作　　者：王爱民[1] 阴桢宏[2] 张莉[1] 马雪梅[2] 宋鑫[1] 王宝恩[1] 

机构地区：[1]解放军252医院消化科,河北保定071000 [2]北京友谊医院肝病研究中心

出　　处：《中华实验外科杂志》2012年第12期2458-2459,共2页Chinese Journal of Experimental Surgery

基　　金：军队“十五”杰出人才基金课题（01J002）;军队“十一五”面上课题（06MA073）

摘　　要：目的观察黄芪对HSC—T6细胞基质分解素-1（MMP3）及其抑制因子基因表达的影响。方法以不同浓度的黄芪（1．0、0．5g/L）作用于培养的肝星状细胞株HSC—T6细胞48h，收集细胞，提取总RNA；用逆转录定量聚合酶链反应（RT—PCR）方法测定MMP3及金属蛋白酶组织抑制因子-1（TIMP-1）的基因表达水平。结果MMP3基因表达水平在黄芪1．0、0．5g/L两组分别为3．36±0．42、2．63±0．36，而空白对照组为2．13±0．30。TIMP．1基因表达水平在黄芪1．0、0．5g/L两组分别为3．96±0．66、4．13±0．50，而空白对照组为4．03±0．56。结论黄芪可明显增强HSC—T6细胞MMP3的基因表达，且与剂量有关；但对TIMP-1的基因表达无明显影响。Objective To investigate the effects of Astragalus on gene expression of stromelysin-1 （MMP3） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in HSC-T6 cells. Methods HSC-T6 cells were cultured in medium containing different concentrations of Astragalus （ 1.0, 0. 5 g/L） for 48 h and harvested. Total RNA of HSC-T6 cells was isolated and gene expression levels of MMP3 and tIMP-1 were measured by using reverse transcription polymerase chain reaction. Results MMP3 gene expression level in different groups of Astragalus （ 1.0, 0. 5 g/L） was 3.36 ± 0. 42, and 2. 63 ± 0. 36 respectively, and that in control group was 2. 13 ± 0. 30. TIMP-1 gene expression level in different groups of Astragalus （ 1.0, 0. 5 g/L） was 3.96 ± 0. 66, and 4. 13 ± 0. 50 respectively, and that in control group was 4. 03±0. 56. Conclusion Astragalus may significantly increase MMP3 gene expression of HSC-T6 cells in a dose- dependent manner. Astragalus has no significant effect on TIMP-1 gene expression.

关 键 词：黄芪 肝星状细胞 基质分解素-1 金属蛋白酶组织抑制因子-1 基因表达 

分 类 号：R575.2[医药卫生—消化系统]