柴胡对肝星状细胞基质分解素-1及Ⅰ型胶原基因表达的影响  

Effects of Radix Bupleuri on gene expression of stromelysin-1 and type I collagen in HSC-T6 cells

作　　者：王爱民[1] 阴桢宏[2] 付晓霞[1] 马雪梅[2] 朱志坚[1] 王宝恩[2] 

机构地区：[1]解放军252医院消化科,河北保定071000 [2]北京友谊医院肝病研究中心

出　　处：《中华实验外科杂志》2012年第12期2467-2468,共2页Chinese Journal of Experimental Surgery

基　　金：军队“十五”杰出人才基金课题（01J002）;军队“十一五”面上课题（06MA073）

摘　　要：目的观察柴胡对HSC—T6细胞基质分解素-1（MMP3）及I型胶原基因表达的影响。方法以不同浓度的柴胡（1．0、0．5g/L）作用于培养的肝星状细胞株HSC—T6细胞48h，收集细胞，提取总RNA；用逆转录定量聚合酶链反应（RT—PCR）方法测定MMP3及I型胶原的基因表达水平。结果MMP3基因表达水平在柴胡1．0、0．5g/L两组分别为2．02±0．29、2．30±0．33，而空白对照组为2．13±0．30。I型胶原基因表达水平在柴胡1．0、0．5g／L两组分别为0．82±0．13、1．11±0．13，而空白对照组为3．12±0．46。结论柴胡可明显抑制HSC—T6细胞I型胶原的基因表达，且与剂量有关；但对基质分解素-1的基因表达无明显影响。Objective To investigate the effects of Radix Bupleuri on gene expression of stromely- sin-1 （MMP3） and type I collagen in HSC-T6 cells. Methods HSC-T6 cells were cultured in medium containing different concentrations of Radix Bupleuri （1.0, 0. 5 g/L） for 48 h and harvested. Total RNA of HSC-T6 cells was isolated and gene expression levels of MMP3 and type I collagen were measured by using reverse transcription polymerase chain reaction. Results MMP3 gene expression level in different groups of Radix Bupleuri （ 1.0, 0. 5 g/L） was 2. 02 ±0. 29 and 2. 30 ±0. 33 respectively, and that in con- trol group was 2. 13 ±0. 30. Type I collagen gene expression level in different groups of Radix Bupleuri （1. 0, 0. 5 g/L） was 0. 82 ± 0. 13 and 1.11 ± 0. 13 respectively, and that in control group was 3.12 ± 0. 46. Conclusion Radix Bupleuri may significantly inhibit type I collagen gene expression in HSC-T6 cells in a dose-dependent manner. Radix Bupleuri has no effect on MMP3 gene expression.

关 键 词：柴胡 肝星状细胞 基质分解素-1 I型胶原 基因表达 

分 类 号：R575.2[医药卫生—消化系统]