人食管鳞癌EC9706细胞线粒体DNA中ND1基因突变的检测及意义  

Detection of Mutation in the Mitochondrial DNA ND1 Gene and Its Significance in the Human Esophageal Squamous Carcinoma EC9706 Cells

作　　者：刘宗文[1] 冯天平[2] 赵景志[2] 田薇薇[1] 韩娜[1] 孙淼淼[3] 娄欣[4] 胡爱侠 

机构地区：[1]郑州大学第二附属医院肿瘤科,河南郑州450014 [2]郑州大学公共卫生学院,河南郑州450001 [3]河南省肿瘤医院病理科,河南郑州450003 [4]郑州市中心医院,河南郑州450052 [5]商丘市中心医院,河南商丘476000

出　　处：《肿瘤基础与临床》2012年第6期461-463,共3页journal of basic and clinical oncology

基　　金：河南省科学技术厅项目(编号:102300410082)

摘　　要：目的通过对人食管鳞癌EC9706细胞线粒体DNA(mtDNA)中ND1基因进行检测,分析其基因突变的意义。方法培养人食管鳞癌EC9706细胞,提取其mtDNA中的ND1基因,并测序。结果测序发现,人食管鳞癌EC9706细胞mtDNA中ND1基因的3 971处出现点突变,由C突变为T。结论人食管鳞癌EC9706细胞中mtDNA的ND1基因的突变与食管癌发生、发展关系密切。Objective To study the mutation of the mitochondrial DNA （mtDNA） ND1 gene of the human e- sophageal squamous carcinoma EC9706 cells. Methods The mtDNA was extracted in human esophageal squamous carcinoma EC9706 cells and ND1 gene was amplified and sequenced. Results The gene sequencing was success- fully detected. Point mutation （ C3 971T） in ND1 gene was found by sequencing in the human esophageal squamous carcinoma EC9706 cells. Conclusion The mutation of the mtDNA ND1 gene of the human esophageal squamous carcinoma EC9706 ceils is related to the occurrence and development of esophageal carcinoma.

关 键 词：人食管鳞癌 EC9706细胞 线粒体DNA ND1基因 

分 类 号：R735.1[医药卫生—肿瘤] R730.23[医药卫生—临床医学]