环境条件对芽孢杆菌B-9987菌株防御酶诱导效应的影响  

Inducing Effect of Defense Enzyme Activities by Bacillus marinus B-9987 under Different Environmental Conditions

作　　者：高伟[1,2] 田黎[1,2] 张久明[2] 郑立[1] 崔志松[1] 史振平[2] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]青岛科技大学生物系,山东青岛266042

出　　处：《植物生理学报》2012年第11期1037-1042,共6页Plant Physiology Journal

基　　金："十二五"国家高技术研究发展计划"863"重大项目(2011AA10A202-2和2011AA09070402);"十二五"国家科技支撑计划(2011BAE06B04);青岛科技计划基础研究项目(10-3-4-4-7-jch);海洋公益项目(201005032-2)

摘　　要：研究室内外条件下,海洋芽孢杆菌不同处理物对番茄防御酶系的诱导作用。分别使用不同浓度芽孢杆菌B-9987发酵液和无细胞滤液喷施番茄植株,于室内外两种环境条件下养殖,测定了处理11d内番茄5种防御酶的变化趋势。两种处理诱导抗性结果显示,在两种环境条件下处理植株苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)、过氧化氢酶(CAT)较空白对照植株酶活性均有不同程度增加。除超氧化物歧化酶(SOD)外,室内环境较室外更利于B-9987菌株处理液对番茄防御酶系的诱导作用发挥;相同环境条件下,发酵液的处理诱导活性较强。B-9987菌株对番茄防御酶系有较好的诱导作用,但诱导作用的发挥受环境条件影响明显。The comparative study on the activities of defense enzymes was obtained in tomato leaves under dif- ferent environmental conditions. The tomato plants were sprayed with B-9987 fermentation broth and cell free filtrate. The activities of the 5 defense enzymes in tomato leaves were assayed. The trend of the enzymes activi- ties in 11 days was analysed in the experiment. According to the result, the B-9987 extracts had an inducing ef- fect on phenylalanine ammonia lyase （PAL）, polyphenol oxidase （PPO）, peroxidase （POD）, catalase （CAT） in tomato leaves. The indoor treatment of the enzymes [except superoxide dismutase （SOD）] showed higher effect comparing outdoor treatment. The inducement of fermentation broth was much better than the cell filtrate. The result showed that the B-9987 extracts had an inducing effect on the defense enzymes in tomato. The environ- mental conditions greatly affect the inducement of B-9987 extracts.

关 键 词：防御酶 海洋芽孢杆菌 诱导抗性 

分 类 号：TQ925[轻工技术与工程—发酵工程]