云南省人乳头状瘤病毒16型E6/E7基因的变异分析  

The Analysis of Human Papillomavirus Type 16 E6/E7 Genetic Variability in Yunnan Province,China

作　　者：杨丽娟[1,2] 岳耀斐 陈俊英[1,2] 潘玥[1,2] 赵玉娇[1,2] 马绍辉[1,2] 孙强明[1,2] 

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650118 [2]云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《病毒学报》2012年第6期645-651,共7页Chinese Journal of Virology

基　　金：国家自然科学基金面上项目(81171946);云南省自然科学基金项目(2009ZC187M);云南省科技厅社会发展科技计划项目(2011CA016)

摘　　要：研究人乳头状瘤病毒16型E6和E7基因在云南省的变异情况。采集获得2 000例妇科门诊样品,提取DNA,以MY09/MY11为外引物,GP5+/GP6+为内引物,采用nest-PCR法对样品HPV-DNA高变区L1区的相应基因进行扩增、测序和分型,筛选得到20例HPV-16型病毒DNA,对其进行E6和E7基因特异性扩增,测序。结果显示20例HPV-16型E6基因中有10例在178位核苷酸发生碱基突变,突变频率为50%,E7基因中有10例在647位核苷酸发生碱基突变,突变频率为50%。进化树分析结果表明在云南省流行的HPV-16型中主要为亚洲变异型,没有发现非洲1型,非洲2型。To investigate E6 and E7 gene variations of human papillomavirus type 16 in Yunnan Province,DNA was extracted from 2000 gynecological outpatient samples.For Human papillomavirus（HPV） genotyping,the genomic DNA was first amplified by the consensus MY09/MY11 primer pair followed by nested PCR with GP5＋/GP6＋ primers,then the PCR products were subjected to direct DNA sequencing.A total of 20 HPV-16 viral DNAs were identified.E6 and E7 genes of HPV-16 viral DNA were then amplified using E6 and E7 specific primers,the PCR products were purified and sequenced.The results showed that mutations were found at nucleotide position 178 of HPV-16 E6 gene in 10 cases,the mutation rate was 50%;For HPV-16 E7 gene,the mutations were found at nucleotide position 647 in 10 cases;the mutation rate was 50%.Phylogenetic analysis showed that Asian（As） variants of HPV-16 were predominated in Yunnan,China.None of African-1,African-2 variants of HPV-16 was found in this region.

关 键 词：子宫颈癌 HPV-16 进化树分析 基因变异 E6基因 E7基因 

分 类 号：R373.9[医药卫生—病原生物学]