牛蒡低聚果糖对脂多糖诱导下RAW264.7细胞炎症模型的抗炎作用  

Anti-inflammatory effect of burdock fructo-oligsaccharide on lipopolysaccharide-stimulated RAW264.7 cell

作　　者：刘晶[1] 潘晓华[2] 宋珍[1] 程梅华[3] 王墨林[1] 

机构地区：[1]山东大学医学院医学遗传学研究所,济南250012 [2]山东大学附属省立医院普通外科,济南250021 [3]济宁市任城区计划生育服务站,山东济宁212100

出　　处：《山东大学学报（医学版）》2012年第12期41-46,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金青年项目(30500190);国家自然科学基金主任基金(31240031);山东省自然科学基金(ZR2012HQ016);山东省优秀中青年科学家奖励基金(2008BS03024)

摘　　要：目的观察牛蒡低聚果糖(BFOS)对RAW264.7炎症细胞模型的抗炎作用。方法用脂多糖(LPS)处理巨噬细胞系RAW264.7细胞,建立炎症模型,并用不同浓度BFOS处理。采用定量PCR检测环氧合酶2(COX-2)、一氧化氮合成酶(iNOS)、单核细胞趋化蛋白1(MCP-1)、白细胞介素6(IL-6)等炎性因子的表达水平,ELISA检测BFOS处理前后MCP-1、IL-6的分泌量。Western blot法检测COX-2、iNOS、κB抑制因子(IκB)、核转录因子κB p65(NF-κBp65)、细胞外信号调节激酶(Erk)、P38、c-Jun氨基末端激酶(JNK)的磷酸化蛋白表达水平。结果与对照组相比,经BFOS处理后,细胞iNOS、COX-2、MCP-1及IL-6的水平表达显著下降(P<0.01)。BFOS处理组p-IκB、p-NF-κBp65、p-Erk、p-P38、p-JNK等蛋白表达较LPS单独处理组显著下降(P<0.01)。结论 BFOS对LPS诱导的RAW264.7细胞炎症模型具有抗炎作用。Objective To investigate the anti-inflammatory effect of burdock fructo-oligsaccharide （BFOS） on the in- flammatory model of RAW264.7 cells. Methods The inflammation model was constructed by treating RAW264.7 cells with lipopolysaccharide （ LPS ）, and treated with different doses of BFOS. Expressions of Cyclooxygenase （ COX- 2）, Nitric oxide synthases （ iNOS）, monocyte chemotactic protein-1 （ MCP-1 ） and Interleukin-6 （IL-6） were detected by real-time PCR. Expressions of MCP-1 and IL-6 were determined by ELISA before and after the treatment with BFOS. Expressions of COX-2, iNOS, phosphorylated IκB, NF-κBp65, extracellular signal-regulated （Erk）, P38 and c-Jun N-terminal kinase （JNK） were detected by Western blot. Results The expression levels of iNOS, COX-2, MCP- 1 and IL-6 of RAW264.7 cells stimulated by LPS increased, and BFOS significantly decreased them. BFOS decreased the expressions of p-IκB,p-NF-κBp65,p-Erk,p-P38, and p-JNK compared to the control. Conclusion BFOS may ex- ert anti-inflammatory effect on RAW264.7 cells stimulated by LPS.

关 键 词：牛蒡低聚果糖 抗炎作用 RAW264 7细胞系 核转录因子ΚB 有丝分裂原活化蛋白激酶 

分 类 号：R394.6[医药卫生—医学遗传学]