白藜芦醇联合TRAIL抑制乳腺癌细胞抗凋亡蛋白Bcl-xl表达  

Synergistic inhibition of Bcl-xl by the combination of Resveratrol and TRAIL in human breast cancer cells

作　　者：仇凤启[1] 孙大鹏[1] 赵丹[1] 刘新莉[1] 李晓曦[1] 马萍[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所二室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2012年第12期2453-2456,共4页Journal of Modern Oncology

摘　　要：目的:探讨白藜芦醇(Resveratrol,RES)联合TRAIL对乳腺癌细胞凋亡及凋亡相关蛋白Bcl-xl表达的影响。方法:50μmol/L RES、100ng/ml TRAIL及二者联合分别处理乳腺癌MDA-MB-231和MCF-7细胞,MTT法检测细胞增殖抑制率,Annexin V-FITC/PI法检测细胞凋亡,Real time PCR检测Bcl-2、Bcl-xl和Mcl-1的mRNA表达,Western blot检测Bcl-xl、Bcl-2和Mcl-1蛋白表达。结果:MTT结果显示RES、TRAIL均能明显抑制乳腺癌细胞增殖,且联合组抑制率高于单独处理组(P<0.05)。RES、TRAIL单独或联合能诱导MDA-MB-231细胞凋亡,凋亡率分别为(16.34±0.34)%,(43.52±0.99)%和(73.60±2.80)%,与对照组的(4.13±0.25)%比较有统计学意义(P<0.05);而对MCF-7凋亡影响不明显。Realtime PCR结果显示RES联合TRAIL能明显抑制MDA-MB-231细胞Bcl-xl mRNA表达,联合组与对照组比较有统计学意义(P<0.05)。Western blot结果显示RES联合TRAIL能抑制Bcl-xl蛋白的表达而对Bcl-2和Mcl-1抑制不明显。结论:RES能增强TRAIL诱导乳腺癌细胞凋亡敏感性,这可能与二者抑制抗凋亡蛋白Bcl-xl的表达有关。Objective：To investigate the synergistic effect of Resveratrol and TRAIL on apoptosis of breast cancer cells and the expression of Bcl-xl.Methods： Human breast cancer cells MDA-MB-231 and MCF-7 were treated with 50umol/L Resveratrol and 100ng/ml TRAIL,alone or in combination.MTT assay was used to detect the cell proliferation and Flow cytometry was applied to analyze cell apoptosis.The expressions of Bcl-xl,Bcl-2 and Mcl-1 in mRNA and protein level were analyzed by real time PCR and Western blot,respectively.Results： Resveratrol and TRAIL alone or in combination significantly inhibits the proliferation of MDA-MB-231 and MCF-7 cells,Annexin V-FITC/PI binding assay showed that Resveratrol and TRAIL alone or in combination induced apoptosis in MDA-MB-231 cells,and the combined group induced higher apoptosis rates[（73.60±2.80）% vs（16.34±0.34）%,（43.52±0.99）%,P0.05],but the effect on MCF-7 was insignificant.The combination of Resveratrol and TRAIL inhibited the expression of Bcl-xl both in mRNA and protein level,significantly.Conclusion： The combination of Resveratrol and TRAIL can enhance the apoptosis of breast cancer cells,the potential mechanism may be via inhibit the expression of Bcl-xl.

关 键 词：白藜芦醇 TRAIL 凋亡 抗凋亡蛋白 BCL-XL 

分 类 号：R733.61[医药卫生—肿瘤] R737.9[医药卫生—临床医学]