雷公藤甲素对人前列腺癌PC-3细胞裸鼠移植瘤模型的作用  

Effect of triptolide on PC-3 cell xenografts in nude mice

作　　者：杜柄承[1] 乔小霞 张斌[1] 雷鸣[1] 马元春[1,3] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]宁夏中医医院,宁夏银川750021 [3]青海卫生职业技术学院,青海西宁810000

出　　处：《现代肿瘤医学》2012年第12期2480-2483,共4页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:61178084);国家林业公益性行业科研专项(编号:201204603)

摘　　要：目的:探讨雷公藤甲素对前列腺癌PC-3细胞裸鼠移植瘤模型的作用。方法:构建前列腺癌PC-3细胞裸鼠皮下移植瘤模型,分为实验组和对照组,实验组给予雷公藤甲素治疗,0.4mg/(kg.d),皮下注射,连用15天,对照组仅给予含有等量DMSO的生理盐水,检测药物处理后肿瘤的变化,并通过免疫荧光染色的方法检测SUMO特异蛋白酶SENP1的表达。结果:雷公藤甲素能够显著抑制移植瘤的增长,并能抑制SENP1的表达水平。结论:雷公藤甲素可以显著抑制PC-3细胞裸鼠移植瘤的增长,并降低SENP1的表达水平。Objective：To investigate the role of triptolide on prostate cancer PC-3 cell xenografts in nude mice.Methods：To costruct prostate cancer PC-3 cells in nude mice subcutaneous xenograft model,divided into experimental and control groups.The experimental group was treated by triptolide,0.4mg/（kg·d）,subcutaneous injection,for 15 days.The control group only received saline containing the same amount of DMSO.To detect the change of tumor after drug treatment,and the SUMO-specific protease SENP1 expression by immunohistochemical methods.Results： Triptolide could significantly inhibit the growth of transplanted tumors,and inhibit SENP1 expression levels.Conclusion： Triptolide can significantly inhibit the growth of PC-3 cell xenografts in nude mice,reduce the level of SENP1 protein expression in prostate cancer.

关 键 词：雷公藤甲素 PC-3细胞 裸鼠移植瘤 

分 类 号：R735.9[医药卫生—肿瘤]