酶联免疫吸附法和凝集素微量离心柱法检测甲胎蛋白异质体的研究  

Clinical research of AFP variant tested with enzyme linked immunosorbent assay and microcentrifugal column method

作　　者：黄彩云[1] 韩素桂[1] 张景华[1] 王晓蕊[2] 李世龙[1] 周秀艳[1] 

机构地区：[1]唐山市人民医院核医学检验科,河北唐山063000 [2]唐山市协和医院,河北唐山063000

出　　处：《现代肿瘤医学》2012年第12期2574-2576,共3页Journal of Modern Oncology

基　　金：河北省科学研究重点课题(编号:20110613)

摘　　要：目的:比较酶联免疫吸附法(enzyme linked immunosorbent,ELISA)和吸附柱微量离心柱法[lens cu-linaris agglutinin(LCA)-coupled spin column,ACSC]检测甲胎蛋白异质体(alpha-fetoprotein isoforms,AFP-L3)在甲胎蛋白(alpha-fetoprotein,AFP)阳性的良恶性肝病鉴别诊断中的价值。方法:采用ELISA法检测92例原发性肝癌(hepatocellular carcinoma,HCC),45例慢性肝炎及肝硬化患者血清中AFP-L3浓度,用ACSC法检测该批标本的AFP-L3/AFP%,比较两种方法对良恶性肝病鉴别诊断的价值。结果:ELISA法检测AFP-L3浓度和ACSC法检测AFP-L3/AFP%对92例原发性肝癌(HCC)的敏感性分别是83.69%和85.86%,对45例慢肝、肝硬化的特异性分别为88.88%和82.22%,ELISA法和ACSC法两种方法诊断原发性肝癌的符合率分别为85.40%和84.67%,在受试者工作特征曲线(recover operatin characteristic,ROC)下的面积AUC分别为0.819和0.823。结论:ELISA法检测AFP-L3浓度和ACSC法检测AFP-L3/AFP%在HCC鉴别诊断中具有相似的临床价值,但是ELISA法操作简便、省时、经济、患者易接受,更利于临床推广应用。Objective：To compare the clinical value of enzyme linked immunosorbent（ELISA） assay method and lens culinaris agglutinin（LCA）-coupled spin column（ACSC） method for the measurement of alpha-fetoprotein variant（AFP-L3） in the diagnosis of hepatocellular carcinoma（HCC）.Methods： Seuum AFP-L3 variants in 92 hepatocellular carcinoma（HCC） patients and 45 chronic and cirrhosis patients were tested by ELISA assay method and the ACSC method.Results： In 92 HCC patients,the sensitivity of AFP-L3 was 83.69% and 85.86% respectively;in 45 chronic and cirrhosis patients,the specificity of AFP-L3 was 88.88% and 82.22% respectively.The diagnostic accuracy was 85.40% and 84.67% respectively.The area under the recover operation characteristic（ROC） curve was 0.819 and 0.823 respectively.Conclusion： Use ELISA method to detect AFP-L3 not only convenient,time saving,but also more economic,it have a high clinical value in the diagnosis of hepatocellular carcinoma（HCC）.

关 键 词：酶联免疫吸附法 凝集素微量离心柱法 甲胎蛋白 甲胎蛋白异质体 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]