小鼠血管内膜增生模型的建立  

Induction of Rapid Intimal Hyperplasia in Mice

作　　者：杨旭[1] 颜新[1] 刘震泽 李俊 吴文婷 王维刚 

机构地区：[1]同济大学中医研究所,上海200092 [2]上海南方模式生物研究中心,上海201203

出　　处：《中国细胞生物学学报》2012年第12期1240-1245,共6页Chinese Journal of Cell Biology

基　　金：国家高技术研究发展计划(836计划;No.2008AA02Z126);上海市科委科技创新行动计划(No.10140901400);上海市科学技术委员会科研计划项目课题(No.10DZ1975600)资助项目~~

摘　　要：该文主要介绍一种可以在小鼠中有效地诱导血管内膜增生的方法。该方法使用硅胶管嵌套小鼠股动脉,造成股动脉血管内膜增生。病理切片观察表明,实验组小鼠股动脉与假手术组相比出现了内膜层和中膜层不规则增厚,管腔狭窄,细胞排列紊乱,内膜炎症细胞浸润等。免疫组织化学染色显示内膜增生部分是由于血管平滑肌细胞(VSMCs)增生和基质积聚所致。该模型的建立对动脉粥样硬化病理机制研究和治疗药物的研发具有重要意义。An approach to induce neointimal growth in mice is presented. A silicone tube collar is placed around the femoral artery of the mouse. After 2 weeks, significant changes such as irregular growth of intima, me- dia thickness and luminal stenosis, increasing of endothelial cell permeability, disturbance of cell alignment and infiltration of inflammatory cells towards intima in collar-treated groups in comparison to sham-operated groups, can be observed in histological sections. It is indicated by immunostaining that the observed neointimal growth is caused by hyperplasia of VSMCs and deposition of matrixes. The present model is therefore of great importance in investigating the pathological mechanisms of atherosclerosis and developing therapeutic drugs.

关 键 词：小鼠疾病模型 内膜增生 外膜炎症 套管 

分 类 号：R-332[医药卫生]