山东小麦黄花叶病毒的分子检测与鉴定  

Molecular Detection and Identification of Wheat Yellow Mosaic Virus in Shandong Province

作　　者：丛倩倩[1] 许斐斐[1] 李向东[1] 张广民[1] 

机构地区：[1]山东农业大学植物保护学院植物病理学系,山东泰安271018

出　　处：《山东农业科学》2012年第11期8-11,共4页Shandong Agricultural Sciences

基　　金：国家行业性公益项目"粮食作物重要病毒防控技术研究与示范

摘　　要：利用RT-PCR对2012年山东10个地区送检的105个小麦样品进行了检测,从泰安、临沂、枣庄3个地区的样品中检测到了小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV)。这3个地区WYMV分离物CP基因的核苷酸一致率为97.6%～98.3%,氨基酸一致率为98.3%～99.0%。在利用CP基因核苷酸序列构建的系统进化树中,这3个分离物均与WYMV分离物聚类到一起,其中枣庄分离物和河南分离物(FR828007)亲缘关系更近。One hundred and five wheat samples collected from 10 districts of Shandong Province were detected for wheat yellow mosaic virus （WYMV） using RT -PCR. WYMV was detected from samples from Taian, Linyi and Zaozhuang. The coat protein genes of WYMV from these three areas shared the nucleotide identity of 97.6% -98.3% and the amino acid identity of 98.3% -99.0%. In the phylogenetic tree constructed based on the nucleotide sequence, the three isolates were clustered together with other WYMV isolates. The isolates from Zaozhuang had closer genetic relationships with the isolate from Henan.

关 键 词：小麦黄花叶病毒 CP基因 系统进化分析 

分 类 号：Q781[生物学—分子生物学]