高效液相色谱法测定四逆泡腾片中甘草黄酮含量  

HPLC determination of licorice flavonoids in Sini effervescent tablets

作　　者：王跃生[1] 李欣晴[2] 饶毅[1] 魏惠珍[1] 胡景婷[3] 刘玲[3] 

机构地区：[1]中药固体制剂制造技术国家工程研究中心,南昌330006 [2]赣州市人民医院,赣州341000 [3]江西中医学院,南昌330004

出　　处：《药物分析杂志》2012年第12期2168-2171,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家重点基础研究发展计划(973计划);编号2010CB530602

摘　　要：目的:建立四逆泡腾片中甘草黄酮的高效液相色谱定量方法。方法:采用四逆泡腾片提取物酸水解方法制备甘草黄酮主要苷元—甘草素与异甘草素,采用Hypersil ODS2 C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱,流速0.8 mL.min-1,检测波长230 nm,柱温40℃。结果:甘草素、异甘草素线性范围分别为0.0219～0.656μg、0.0216～0.647μg,r均为0.9999;平均加样回收率以甘草素与异甘草素的量之和计为100.6%,RSD为1.5%(n=6)。结论:该方法专属,分离效果好,灵敏度高,可用于四逆泡腾片的质量控制。Objective：To establish an HPLC for determination of licorice flavonoids in Sini effervescent tablets Methods ： An HPLC method was applied to separate and qualify the main components of licorice flavonoids,liquiriti- genin and isoliquiritigenin after acid hydrolysis was performed on these extracts. The samples were separated by a Hypersil ODS2 Cls column（4. 6 mm ×250mm,5μm） using acetouitrile -water as the mobile phase for gradient e- lution at a flow rate of 0. 8 mL · min-l,detection wavelength 230 nm,the column temperature 40 ℃. Results：The calibration curves were linear in the range of 0. 0219 - 0. 656 μg （ r = 0. 9999） for liquiritigenin, and 0. 0216 - 0. 647 g（ r = 0. 9999 ） for isoliquiritigenin; The sum of average recovery for liquiritigenin and isoliquiritigenin was 100.6%, and RSD was 1.5% （ n = 6）. Conclusion：The HPLC method developed for simultaneous determination of liquiritigenin and isoliquiritigenin is specific with good separation effect and high sensitivity which can be used for the quality control of Sini effervescent tablets.

关 键 词：四逆泡腾片 甘草黄酮 二氢黄酮类 查尔酮类化合物 甘草素 异甘草素 酸水解 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]