重金属Pb^(2+)与蛋白质多组份体系的结合反应机制研究  

Interaction between Pb^(2+) and Multi-component Protein System

作　　者：郭明[1] 何玲[1] 

机构地区：[1]浙江农林大学化学系,浙江杭州311300

出　　处：《环境科学与技术》2012年第12期33-39,81,共8页Environmental Science & Technology

基　　金：国家自然科学基金项目(20877072);浙江省科技创新项目资助(2010R412042)

摘　　要：滴涂法制备壳聚糖修饰电极(Chitosan/Glassy Carbon Electrode,CTS/GCE),并通过循环伏安法和电化学交流阻抗法表征修饰电极;利用修饰电极研究Pb2+离子与血清蛋白质混合体系及单组分体系的结合反应机理,并进行分析比较。结果表明,CTS/GCE修饰电极测定Pb2+-蛋白质分子相互作用时显现良好的线性响应关系,利用建议的理论方程计算了Pb2+与蛋白质的结合参数;分析实验数据表明混合组分体系中蛋白质分子间也存在相互作用,从而影响Pb2+与单组分蛋白质分子相互作用,Pb2+与多组分蛋白质溶液体系的相互作用参数不等于Pb2+与单组分蛋白质分子相互作用参数之和;同时,文章提出了一个蛋白质-蛋白质分子相互作用的计算公式,以此定量衡量多组分蛋白质混合体系中蛋白质分子间的相互作用。研究结果可为研究重金属离子与蛋白质组的相互作用提供前期基础,并为蛋白质-蛋白质分子间相互作用研究提供新方法。Electrochemical method has been applied in studying mechanism of interaction between metal ions and protein molecule, in which electrodes are applied. In this paper, a chitosan-modified electrode was prepared through drop method and characterized by cyclic voltammetry and electrochemical impedance spectroscopy, which was used to probe into the mechanisms of interaction between Pb2＋ and the systems of mixed protein and single component protein, suggesting a good linear response. The binding parameters of Pb2＋ with protein were calculated by a theoretical equation and the experimental data indicated that in the naixed system existed the interaction between protein and protein molecules as well, thus affecting the interaction with single protein molecule. As a result, a calculation formula for measuring protein-protein interaction was proposed, which can be used to quantitatively measure the interaction of protein in a multi-component system.

关 键 词：PB2+ 蛋白质混合体系 壳聚糖修饰电极 相互作用 电化学方法 

分 类 号：X132[环境科学与工程—环境科学]