镉对小鼠骨生物力学及成骨细胞MG-63的影响  

Effects of cadmium on bone biomechanics in mice and osteoblast MG-63

作　　者：黄泽昊[1,2] 侯艳丽[3] 李君平[3] 王立新[2] 张昌文[2] 王小岩[2] 荣海钦[3] 任建武[1] 曲广波[2] 

机构地区：[1]北京林业大学,北京100083 [2]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [3]山东省内分泌与代谢病研究所,济南250062

出　　处：《环境化学》2012年第12期1835-1839,共5页Environmental Chemistry

基　　金：国家自然基金(21077128,20921063,21177151)资助

摘　　要：为了探讨镉对骨的影响及机制,选取7周龄C57BL/6J小鼠给予氯化镉2 mg.kg-1腹腔注射,每周2次,染毒4周后进行了骨力学参数的测量.结果显示,暴露组股骨的最大弯曲应力及弹性模量降低,骨灰干重比下降.体外结果显示,氯化镉(CdCl2)的暴露影响人成骨肉瘤细胞MG-63的细胞形态、活力及增殖,并且呈剂量依赖性.因此,氯化镉暴露可导致小鼠骨量下降和骨力学性能下降,并对成骨细胞有明显的毒性.Cadmium is one of the most widely distributed heavy metals in the environment. In this study, C57BL/6 J mice were injected intraperitoneally with cadmium chloride at 2 mg .kg-1 twice per week. After 4week exposure, femur was taken out for 3-point-bending test and ashes-dry weight ratio evaluation. The maximal bending stress, elastic modulus and ashes-dry weight ratio of the femur were lower in the exposuregroup than that in the control one. Cadmium also affected the morphology, activity and proliferation of osteoblast-like ceils MG-63 in a dose-dependent manner. Our results suggest that cadmium exposure inducesthe decrease in bone mass and biomechanics in mice, and obvious toxicity to osteoblasts.

关 键 词：镉 骨质疏松 成骨细胞 

分 类 号：X174[环境科学与工程—环境科学]