重组氨基酰化酶1与N-乙酰鸟氨酸脱乙酰酶酶学性质比较  

Comparison of Enzymatic Properties Between Aminoacylase 1 and N-acetyl-ornithine Deacetylase

作　　者：高晗[1] 徐礼生[1] 刘均忠[1] 刘茜[1] 焦庆才[1] 

机构地区：[1]南京大学生命科学学院医药生物技术国家重点实验室,江苏南京210093

出　　处：《精细化工》2012年第12期1186-1189,1216,共5页Fine Chemicals

基　　金：国家技术创新基金(02CJ-13-01-16)~~

摘　　要：利用基因工程手段重组表达了牛肾来源的氨基酰化酶1(ACY1),并与实验室之前构建的N-乙酰鸟氨酸脱乙酰酶(NAO)进行比较,以N-乙酰-DL-蛋氨酸为底物,研究了其酶学性质,考察了pH、温度、反应时间、表面活性剂、金属离子等因素对拆分DL-蛋氨酸的影响。结果表明,重组NAO和ACY1拆分300 mmol/L的N-乙酰-DL-蛋氨酸所需时间分别是2 h和6 h;重组NAO的酶活及N-乙酰-L-蛋氨酸的转化率分别为1 139.41 U/g和98%,高于重组ACY1的671.24 U/g和58.78%,约是重组ACY1的1.7倍。Aminoacylase I（ACY1 ） from bovine kidney was recombinantly expressed in E. coli BL21 （ DE3 ） with the vector pETDuet. The enzymatic properties of ACY1 and N-acetyl-ornithine deacetylase （NAO） were compared and studied. Several influencing factors of the enzyme reaction, such as pH, temperature ,enzyme reaction time, surfactant and metal irons, were investigated. The results indicate that the resolution of 300 mmol/L N-Ac-DL-methionine with ACY1 needs 6 h, while that with NAO needs 2 h. The enzyme activity and conversion rate of NAO（ 1 139.41 U/g and 98% ） are about 1.7 times higher than those of ACY1 （671.24U/g and 58.78% ）.

关 键 词：重组N-乙酰鸟氨酸脱乙酰酶 重组牛肾来源的氨基酰化酶1 拆分 蛋氨酸 生物工程 

分 类 号：Q55[生物学—生物化学]