蔬菜中低磷脂肪酸及乳酸的气相色谱分析  

Analysis Of C_1～ C_8 Fatty Acids and Lactic Acid in Vegetables by Gas Chromatography

作　　者：卿笑天[1] 刘红鸣[1] 张波[1] 

机构地区：[1]川北医学院化学教研室,南充637007

出　　处：《分析化学》2000年第7期842-845,共4页Chinese Journal of Analytical Chemistry

摘　　要：使用４’－胆甾烯氧羰基苯并－１５－冠－５（简称ＣＨ－Ｋ１５－Ｃ－５）气相色谱固定相建立了蔬菜中Ｃ１－Ｃ８脂肪酸及乳酸的定性、定量分析方法，测定的线性范围为０．１０～５０ｍｇ／Ｌ，ＲＳＤ≤３．７６％，回收率≥ ９７．０５％，该法快速、灵敏、准确，具有实用价值。The esterifications of fatty acids and lactic acid in the presence of N, N-dimethylformamid was carried out. The separation of the products was accomplished on the column packed with 10% 4'cholestenoxycarbonyl-benzo- 15 - crown-5 (CH- B- C-5 ) liquid crystal crown ether at 130℃. These results could be used for the determination of C1 -C8 fatty acids and lactic acid in vegetables by crown ether liquid crystal gas-liquid chromatography. Retention time and internal standard method were used for qualitative and quantitative analysis, respectively. The standard curve of paeonilorin was y = 170038x + 2815(r = 0.9996), The recovery was 97. 05% (RSD < 3. 8, n = 15). Good linearity was obtained in the concentration range of 0. 10 ~ 50 mg/L. This method is easy and fast to operate, and the result was satisfactory.

关 键 词：低碳脂肪酸 乳酸 蔬菜 气相色谱 分析 夜晶冠醚 

分 类 号：O657.7[理学—分析化学]