全自动缺氧动物模型饲养箱的研制和应用  

Development and application for the automatic hypoxic animal feeding chamber

作　　者：陈豫钦[1] 江倩[1] 云昕[1] 赵磊[1] 付欣[1] 王宁[1] 叶婧美[1] 卢文菊[1] 王健[1] 

机构地区：[1]呼吸疾病国家重点实验室(广州医学院)广州医学院第一附属医院广州呼吸疾病研究所,510120

出　　处：《国际呼吸杂志》2012年第23期1799-1803,F0003,共6页International Journal of Respiration

基　　金：广东省国际合作项目2009B050700041;广州医学院青年项目2010A10

摘　　要：目的为建立符合低氧性肺动脉高压（CHPH）的实验动物模型，设计制造全自动控制的常压持续性低氧动物饲养舱。方法①将氧气浓度控制系统、箱体换气系统、低氧报警系统及缺氧箱箱体系统四个相互独立的功能系统进行有机整合，通过软件程序的集成控制，制作一种自动化程度高，操作简单，实验成本低，系统稳定的全自动缺氧动物培养箱，通过设定使箱体氧浓度稳定在10％左右，箱体内其他指标符合国家SPF动物饲养标准。②10只SD大鼠随机分为低氧组和常氧对照组，每组各5只。低氧组大鼠饲养于全自动动物模型饲养箱，设定好参数，低氧箱放置于SPF环境内，饲养三周。常氧对照组大鼠置于同一间SPF动物房内给予相同饮食饲养，观测大鼠体质量、平均右心室压（MRVP）、右心室收缩压（RVSP）、右心室肥厚指数[RV／（LV＋S）]，并观测肺血管病理学改变。结果缺氧组大鼠体质量增长缓慢，MRVP、RVSP、RV／（LV＋S）明显高于对照组（P〈0．01）。肺组织病理检查示缺氧组大鼠肺内血管管壁明显增厚。结论研制的缺氧动物模型饲养箱自动化程度高，应用方便，调节灵活，结构简单，性能稳定，低成本，易操作。能复制出比较符合CHPH病理生理变化特征的大鼠模型，并能满足其他缺氧性动物实验的需要。Objective To establish the experimental animal models of chronic hypoxic pulmonary hypertension （CHPH）, sustained hypoxia animal feeding tank with automatic control under the atmospheric pressure was designed and manufactured. Methods ①The tank contained four independent systems- -oxygen concentration control system,tank ventilation system,hypoxia alarm system and tank enclosure system. Through software integrated control,this automatic hypoxic animal tank was featured by high degree of automation, simple operation, low cost, and stable system. The oxygen concentration in the tank was stabilized at about 10% by setting, and the other indicators in it were in accord with national SPF animal husbandry standards. ② 10 SD rats were randomly divided into the hypoxic group and the normoxie control group （ n = 5）. Those in the hypoxic group had been raised in this automatic animal models tank with set parameters in SPF environment for three weeks, meanwhile, those in normoxic control group were placed in the same SPF animal room and given the same diet. Body weight of rats, the mean right ventricular pressure （MRVP）, right ventricular systolic pressure （RVSP）, right ventricular hypertrophy index [RV/（LV＋S）] and pulmonary vascular pathological changes were observed. Results Rats in hypoxic group gain weight slowly, and MRVP, RVSP, RV/（LV＋S） were significantly higher than those in the control group （ P〈0.01）. The lung pathology examination revealed the intrapulmonary vascular wall of rats in hypoxic group was significantly thicker. Conclusions This hypoxia animal feeding tank are in high degree of automation, convenient application, flexible adjustment, simple structure, stable performance, low cost, and easy to operate, which can establish the CHPH rats model well in line with the CHPH pathophysiological changes, and meet the other hypoxie animal experiments needs.

关 键 词：低氧性肺动脉高压 动物模型 缺氧 大鼠 

分 类 号：R-332[医药卫生]