糖多孢红霉菌bldD基因提高活跃链霉菌诺西肽产量的研究  

Effects of bldD gene from Saccharopolyspora erythraea on nosiheptide production in Streptomyces actuosus

作　　者：秦汉俊[1] 黄训端[1] 袁莉[1] 刘瑞华 朱林[1] 张部昌[1] 

机构地区：[1]安徽大学生命科学学院,合肥230601 [2]安徽省皖北药业集团股份有限公司,安徽宿州234000

出　　处：《军事医学》2012年第11期847-850,共4页Military Medical Sciences

基　　金：安徽省自然科学基金资助项目(1208085MC46);安徽省教育厅自然科学基金重点资助项目(KJ2009A041)

摘　　要：目的将糖多孢红霉菌bldD基因转入活跃链霉菌(Streptomyces actuosus)N-H,探讨其对诺西肽产量的影响。方法将bldD基因连接到链霉菌整合型表达载体pZMW上构建pZMW-bldD质粒,利用PEG介导的原生质体转化方法将重组质粒pZMW-bldD及对照质粒pZMW-vgb分别导入活跃链霉菌,通过对枯草芽孢杆菌抑菌试验和分光光度计法测定发酵液中诺西肽产量。结果成功构建了活跃链霉菌N-H/pZMW-bldD工程菌株及对照菌株N-H/pZMW-vgb。与出发菌株N-H相比,N-H/pZMW-bldD菌株诺西肽产量提高了68%;与对照菌株N-H/pZMW-vgb相比,N-H/pZMW-bldD菌株诺西肽产量提高了19%。结论在活跃链霉菌中导入糖多孢红霉菌bldD基因可以提高诺西肽产量,其作用效果较vgb基因更为明显,说明bldD基因对抗生素产量的提高具有广谱性。Objective To explore the effect of the bldD gene from Saccharopolyspora erythraea on nosiheptide produc- tion by transferring the bldD gene into Streptomyces actuosus. Methods The bldD gene was connected to the integration expression plasmid pZMW to construct expression plasmid pZMW-bldD before the recombinant plasmid pZMW-bldD and the control plasmid pZMW-vgb were introduced into S. actuosus to construct the strains respectively by means of the PEG-media- ted protoplast transformation. The output of nosiheptide in the original strain and the engineered strains was determined by the inhibition test of Bacillus subtilis and spectrophotometer measurement. Results The engneered strain N,.H/pZMW-bldD and the control strain N-H/pZMW-vgb were successfully constructed. The N-H/pZMW-bldD strain could increase the nosi- heptide productivity by 68% compared with the original strain S. actuosus N-H and by 19% compared with the control strain N-H/pZMW-vgb. Conclusion High yield nosiheptide strain could be gained by transferring the bldD gene and vgb gene into S. actuosus, and bldD gene plays a much more significant role. The bldD gene can improve antibiotics production extensively.

关 键 词：活跃链霉菌 糖多孢菌属bldD基因 诺西肽 抗菌药 

分 类 号：R915[医药卫生—微生物与生化药学]