微波消解衍生化气相色谱-质谱法测定鲨鱼软骨中的脂肪酸  

Determination of Fatty Acids in Shark Cartilage by GC-MS Using Microwave-Assisted Digestion and Derivatization

作　　者：李攻科[1] 何小青[1] 张展霞[1] 

机构地区：[1]中山大学化学系,广东广州510275

出　　处：《色谱》2000年第4期337-339,共3页Chinese Journal of Chromatography

基　　金：广东省自然科学基金博士启动基金! ( 9740 78);教育部留学回国人员科研启动基金! ( 4 1 540 9)资助项目

摘　　要：研究了用微波技术消解衍生化气相色谱 质谱法 (GC MS)快速测定鲨鱼软骨中脂肪酸的分析方法。用正交设计试验优化消解衍生化条件 ,以盐酸 甲醇 (体积比为 1∶4)体系作消解衍生化溶液 ,在 60 0W微波功率作用下加热 4min进行样品的前处理 ,将样品的消解、脂肪酸的衍生化及脂肪酸甲酯的萃取融于一体。方法具有灵敏度高、省时、省试剂、操作方便等特点。该法适用于大批量固体样品中脂肪酸的测定。A rapid microwave assisted digestion and derivatization method for the determination of fatty acids in shark cartilage by GC MS was developed The optimum conditions for digestion and derivatization were studied in detail using orthogonal design The digestion and derivatization were accomplished in 4 minutes at 600 W microwave power using HCl methanol (1∶4, V/V) as digestion and derivatization solvent, and the extraction of the target analytes could be carried out simultaneously This method is rapid, solvent saving, and particularly suitable for the rapid determination of fatty acids in solid samples

关 键 词：微波消解衍生化 鲨鱼软骨 脂肪酸 GC-MS 

分 类 号：Q959.42[生物学—动物学] Q547.03