一种疏水色谱填料的特性及应用的研究  

Study on the Characteristics and Applications of a New Hydrophobic Interaction Chromatographic Packing Using Chitosan as Support

作　　者：王云[1] 郭敏亮[1] 姜守磊[1] 陈天[1] 姜涌明[1] 陈云[1] 

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《色谱》2000年第4期354-356,共3页Chinese Journal of Chromatography

基　　金：江苏省科委"九五"农业重点攻关项目! (BE96443)

摘　　要：以交联壳聚糖为基质 ,正戊醛为配基 ,利用改进的方法制备了疏水作用色谱 (HIC)填料 ,并对该色谱填料的吸附行为和应用作了研究。结果表明 ,此类填料对蛋白质的吸附行为符合疏水相互作用理论 ,对α 淀粉酶的纯化活性回收率大于 80 %。Based on chitosan,by using valeraldehyd as ligand,a new hydrophobic interaction chromatographic (HIC) packing material was prepared by an improved method.The effects of salt concentration, temperature,pH on the adsorption of bovine serum albumin(BSA) were investigated.The results showed that the adsorption behavior of protein by packing material is in agree with the theory of hydrophobic interaction.The α amylase could be separated by HIC with high recovery of enzymatic activity.

关 键 词：疏水作用色谱 填料 壳聚糖 吸附行为 

分 类 号：O657.7[理学—分析化学]